Carcamo,E. Roldan-Salgado,A. Osuna,J. Bello San-Martin,I. Yanez,J.A. Saab-Rincon,G. Viadiu,H. Gaytan,P. 2017. Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly ACS Omega, 2, 3183-3191.

Rodriguez-Mejia,J.L. Roldan-Salgado,A. Osuna,J. Merino,E. Gaytan,P. 2017. A Codon Deletion at the Beginning of Green Fluorescent Protein Genes Enhances Protein Expression Journal of Molecular Microbiology and Biotechnology, 27, 1-10.

Gonzalez-Bacerio,J. Osuna,J. Ponce,A. Fando,R. Figarella,K. Mendez,Y. Charli,J.L. Chavez,M.D. 2014. High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase Protein Expression and Purification, 104, 103-114.

Mendez,Y. Perez-Labrada,K. Gonzalez-Bacerio,J. Valdes,G. Chavez,M.D.A. Osuna,J. Charli,J.L. Pascual,I. Rivera,D.G. 2014. Combinatorial Multicomponent Access to Natural-Products-Inspired Peptidomimetics: Discovery of Selective Inhibitors of Microbial Metallo-aminopeptidases ChemMedChem, 9, 2351-2359.

Osuna,J. Flores,H. Gaytan,P. 2012. A reporter system that discriminates EF-hand-sensor motifs from signal-modulators at the single-motif level FEBS Letters, 586, 3398-3403.

Osuna,J. Flores,H. Saab-Rincon,G. 2012. The beta1 domain of protein G can replace the chorismate mutase domain of the T-protein FEBS Letters, 586, 466-471.

Cote-Velez,A. Perez-Maldonado,A. Osuna,J. Barrera,B. Charli,J.L. Joseph-Bravo,P. 2011. Creb and Sp/Kruppel response elements cooperate to control rat TRH gene transcription in response to cAMP Biochimica et Biophysica Acta (BBA), 1809, 191-199.

Espino-Solis,G.P. Osuna-Quintero,J. Possani,L.D. 2008. Molecular cloning and characterization of the alphaX subunit from CD11c/CD18 horse Veterinary Immunology and Immunopathology, 122, 326-334.

Vazquez-Limon,C. Vega-Badillo,J. Martinez,A. Espinosa-Molina,G. Gosset,G. Soberon,X. Lopez-Munguia,A. Osuna,J. 2007. Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD(+) regeneration Biotechnology Letters, 29, 1857-1863.

Flores-Ramirez,G. Rivera,M. Morales-Pablos,A. Osuna,J. Soberon,X. Gaytan,P. 2007. The effect of amino acid deletions and substitutions in the longest loop of GFP BMC Chemical Biology, 7, 1.

Monroy-Lagos,O. Soberon,X. Gaytan,P. Osuna,J. 2006. Improvement of an unusual twin-arginine transporter leader Peptide by a codon-based randomization approach Applied and Environmental Microbiology, 72, 3797-3801.

Chavez-Gutierrez,L. Matta-Camacho,E. Osuna,J. Horjales,E. Joseph-Bravo,P. Maigret,B. Charli,J.L. 2006. Homology modeling and site directed mutagenesis of pyroglutamyl peptidase II. Insights into omega versus aminopeptidase specificity in the M1 family Journal of Biological Chemistry, 281, 18581-18590.

Yanez,J. Arguello,M. Osuna,J. Soberon,X. Gaytan,P. 2004. Combinatorial codon-based amino acid substitutions Nucleic Acids Research, 32, e158.

Osuna,J. Yanez,J. Soberon,X. Gaytan,P. 2004. Protein evolution by codon-based random deletions Nucleic Acids Research, 32, e136.

Baez-Viveros,J.L. Osuna,J. Hernandez-Chavez,G. Soberon,X. Bolivar,F. Gosset,G. 2004. Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli Biotechnology and Bioengineering, 87, 516-524.

Flores,G. Soberon,X. Osuna,J. 2004. Production of a fully functional, permuted single-chain penicillin G acylase Protein Science, 13, 1677-1683.

Gaytan,P. Osuna,J. Soberon,X. 2002. Novel ceftazidime-resistance beta-lactamases generated by a codon-based mutagenesis method and selection Nucleic Acids Research, 30, e84-e84.

Osuna,J. Perez-Blancas,A. Soberon,X. 2002. Improving a circularly permuted TEM-1 beta-lactamase by directed evolution Protein Engineering, 15, 463-470.

Saab-Rincon,G. Juarez,V.R. Osuna,J. Sanchez,F. Soberon,X. 2001. Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution Protein Engineering, 14, 149-155.

Garcia,J.L. Nunez,C.J. Gonzalez,E.G. Osuna,J. Soberon,X. Galindo,E. 1998. Microbial sensor for new-generation cephalosporins based in a protein-engineered beta-lactamase Applied Biochemistry and Biotechnology, 73, 243-256.

Galindo,E. Lagunas,F. Osuna,J. Soberon,X. Garcia,J.L. 1998. A microbial biosensor for 6-aminopenicillanic acid Enzyme and Microbial Technology, 23, 331-334.

Osuna,J. Soberon,X. Morett,E. 1997. A proposed architecture for the central domain of the bacterial enhancer-binding proteins based on secondary structure prediction and fold recognition Protein Science, 6, 543-555.

Hubbard,T. Tramontano,A. Barton,G. Jones,D. Sippl,M. Valencia,A. Lesk,A. Moult,J. Rost,B. Sander,C. Schneider,R. Lahm,A. Leplae,R. Buta,C. Eisenstein,M. Fjellstrom,O. Floeckner,H. Grossmann,J.G. Hansen,J. Citterich,M.H. Joergensen,F.S. Marchler-Bauer,A. Osuna,J. Park,J. Reinhardt,A. de-Pouplana,L.R. Rojo-Dominguez,A. Saudek,V. Sinclair,J. Sturrock,S. Venclovas,C. Vinals,C. 1996. Update on protein structure prediction: Results of the 1995 IRBM workshop Folding & Design, 1, R55-R63 * .

Osuna,J. Viadiu,H. Fink,A.L. Soberon,X. 1995. Substitution of Asp for Asn at position 132 in the active site of TEM beta-lactamase. Activity toward different substrates and effects of neighboring residues Journal of Biological Chemistry, 270, 775-780.

Viadiu,H. Osuna,J. Fink,A.L. Soberon,X. 1995. A new TEM beta-lactamase double mutant with broadened specificity reveals substrate-dependent functional interactions Journal of Biological Chemistry, 270, 781-787.

Flores,H. Osuna,J. Heitman,J. Soberon,X. 1995. Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants Gene, 157, 295-301.

Osuna,J. Flores,H. Soberon,X. 1994. Microbial systems and directed evolution of protein activities Critical Reviews in Microbiology, 20, 107-116.

Del Rio,G. Osuna,J. Soberon,X. 1994. Combinatorial libraries of proteins: analysis of efficiency of mutagenesis techniques.Biotechniques, 17, 1132-1139.

Merino,E. Osuna,J. Bolivar,F. Soberon,X. 1992. A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.Biotechniques, 12, 508-510.

Osuna,J. Flores,H. Soberon,X. 1991. Combinatorial mutagenesis of three major groove-contacting residues of EcoRI: single and double amino acid replacements retaining methyltransferase-sensitive activities Gene, 106, 7-12.