IBT-UNAM : Dr. Guillermo Gosset Lagarda
Dr. Guillermo Gosset Lagarda Grupo
Departamento de Ingeniería Celular y Biocatálisis
Líneas de investigación
email: gosset @ibt.unam.mx
Teléfonos
Oficina +52 (777) 329-1648 red UNAM 27648 +52 (777) 311-4900 ext 217 Laboratorio +52 (777) 329-1601 red UNAM 27601 +52 (777) 311-4900 ext 217
- Licenciatura: Biología, Escuela de Biología, Guadalajara, Jal. (1987)
Estudiantes Biol. Ernesto Mendoza
Q.B.P. Juan Carlos Fragoso
IBQ. Rodrigo Barrios "Producción de un anticuerpo humano capaz de neutralizar toxinas de alacranes mexicanos en Escherichia coli."
Biol. Jimena Barrientos
Lic. Joel Alcalá
Frances Alejandra Ortiz
Lic. José Ignacio Rodríguez
IBQ. Dante Nestor Perez "Expresión de la proteína recombinante Mgfp-5 de Mytilus galloprovincialis en Escherichia coli."
Biol. Emily Garcia
Publicaciones recientes
Sierra-Ibarra,E. Leal-Reyes,L.J. Huerta-Beristain,G. Hernandez-Orihuela,A.L. Gosset,G. Martinez-Antonio,A. Martinez,A.
2021. Limited oxygen conditions as an approach to scale-up and improve D and L-lactic acid production in mineral media and avocado seed hydrolysates with metabolically engineered Escherichia coli Bioprocess and Biosystems Engineering, 44, 379-389.
Carmona,S.B. Flores,N. Martinez-Romero,E. Gosset,G. Bolivar,F. Escalante,A.
2020. Evolution of an Escherichia coli PTS(-) strain: a study of reproducibility and dynamics of an adaptive evolutive process Applied Microbiology and Biotechnology, 104, 9309-9325.
Martinez,L.M. Martinez,A. Gosset,G.
2019. Production of Melanins With Recombinant Microorganisms Frontiers in Bioengineering and Biotechnology, 7, 285.
Fernandez-Sandoval,M.T. Galindez-Mayer,J. Bolivar,F. Gosset,G. Ramirez,O.T. Martinez,A.
2019. Xylose-glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions Microbial Cell Factories, 18, 145.
Salcedo-Vite,K. Sigala,J.C. Segura,D. Gosset,G. Martinez,A.
2019. Acinetobacter baylyi ADP1 growth performance and lipid accumulation on different carbon sources Applied Microbiology and Biotechnology, 103, 6217-6229.
Sassi,H. Nguyen,T.M. Telek,S. Gosset,G. Grunberger,A. Delvigne,F.
2019. Segregostat: a novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria Microbial Biotechnology, 12, 1064-1075.
Fragoso-Jimenez,J.C. Baert,J. Nguyen,T.M. Liu,W. Sassi,H. Goormaghtigh,F. Van Melderen L. Gaytan,P. Hernandez-Chavez,G. Martinez,A. Delvigne,F. Gosset,G.
2019. Growth-dependent recombinant product formation kinetics can be reproduced through engineering of glucose transport and is prone to phenotypic heterogeneity Microbial Cell Factories, 18, 26.
Hernandez-Chavez,G. Martinez,A. Gosset,G.
2019. Metabolic engineering strategies for caffeic acid production in Escherichia coli Electronic Journal of Biotechnology, 38, 19-26.
Martinez,J.A. Rodriguez,A. Moreno,F. Flores,N. Lara,A.R. Ramirez,O.T. Gosset,G. Bolivar,F.
2018. Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production BMC Systems Biology, 12, 102.
Salas-Navarrete,C. Hernandez-Chavez,G. Flores,N. Martinez,L.M. Martinez,A. Bolivar,F. Barona-Gomez,F. Gosset,G.
2018. Increasing pinosylvin production in Escherichia coli by reducing the expression level of the gene fabI-encoded enoyl-acyl carrier protein reductase Electronic Journal of Biotechnology, 33, 11-16.
Rodriguez,A. Martinez,J.A. Millard,P. Gosset,G. Portais,J.C. Letisse,F. Bolivar,F.
2017. Plasmid-encoded biosynthetic genes alleviate metabolic disadvantages while increasing glucose conversion to shikimate in an engineered Escherichia coli strain Biotechnology and Bioengineering, 114, 1319-1330.
Huerta-Beristain,G. Cabrera-Ruiz,R. Hernandez-Chavez,G. Bolivar,F. Gosset,G. Martinez,A.
2017. Metabolic engineering and adaptive evolution of Escherichia coli KO11 for ethanol production through the Entner-Doudoroff and the pentose phosphate pathways Journal of Chemical Technology and Biotechnology, 92, 990-996.
Garcia,S. Flores,N. de Anda,R. Hernandez,G. Gosset,G. Bolivar,F. Escalante,A.
2017. The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS- Strain of Escherichia coli Journal of Molecular Microbiology and Biotechnology, 27, 11-21.
Fernandez-Sandoval,M.T. Galindez-Mayer,J. Moss-Acosta,C.L. Gosset,G. Martinez,A.
2017. Volumetric oxygen transfer coefficient as a means of improving volumetric ethanol productivity and a criterion for scaling up ethanol production with Escherichia coli Journal of Chemical Technology & Biotechnology, 92, 981-989.
Baert,J. Delepierre,A. Telek,S. Fickers,P. Toye,D. Delamotte,A. Lara,A.R. Jaen,K.E. Gosset,G. Jensen,P.R. Delvigne,F.
2016. Microbial population heterogeneity versus bioreactor heterogeneity: Evaluation of Redox Sensor Green as an exogenous metabolic biosensor Engineering in Life Sciences, 16, 643-651.
Camacho-Zaragoza,J.M. Hernandez-Chavez,G. Moreno-Avitia,F. Ramirez-Iniguez,R. Martinez,A. Bolivar,F. Gosset,G.
2016. Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol Microbial Cell Factories, 15, 163.
Mejia-Caballero,A. de Anda,R. Hernandez-Chavez,G. Rogg,S. Martinez,A. Bolivar,F. Castano,V.M. Gosset,G.
2016. Biosynthesis of catechol melanin from glycerol employing metabolically engineered Escherichia coli Microbial Cell Factories, 15, 161.
Utrilla,J. Vargas-Tah,A. Trujillo-Martinez,B. Gosset,G. Martinez,A.
2016. Production of d-lactate from sugarcane bagasse and corn stover hydrolysates using metabolic engineered Escherichia coli strains Bioresource Technology, 220, 208-214.
Vargas-Tah,A. Moss-Acosta,C.L. Trujillo-Martinez,B. Tiessen,A. Lozoya-Gloria,E. Orencio-Trejo,M. Gosset,G. Martinez,A.
2015. Non-severe thermochemical hydrolysis of stover from white corn and sequential enzymatic saccharification and fermentation to ethanol Bioresource Technology, 198, 611-618.
Vargas-Tah,A. Gosset,G.
2015. Production of Cinnamic and p-Hydroxycinnamic Acids in Engineered Microbes Frontiers in Bioengineering and Biotechnology, 3, 116.
Carmona,S.B. Moreno,F. Bolivar,F. Gosset,G. Escalante,A.
2015. Inactivation of the PTS as a Strategy to Engineer the Production of Aromatic Metabolites in Escherichia coli Journal of Molecular Microbiology and Biotechnology, 25, 195-208.
Vargas-Tah,A. Martinez,L. Hernandez-Chavez,G. Rocha,M. Martinez,A. Bolivar,F. Gosset,G.
2015. Production of cinnamic and p -hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli Microbial Cell Factories, 14, 6.
Balderas-Hernandez,V.E. Trevino-Quintanilla,L.G. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G.
2014. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1 Microbial Cell Factories, 13, 136.
Rodriguez,A. Martinez,J.A. Flores,N. Escalante,A. Gosset,G. Bolivar,F.
2014. Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds Microbial Cell Factories, 13, 126.
Munoz-Gutierrez,I. Moss-Acosta,C. Trujillo-Martinez,B. Gosset,G. Martinez,A.
2014. Ag43-mediated display of a thermostable ß-glucosidase in Escherichia coli and its use for simultaneous saccharification and fermentation at high temperatures. Microbial Cell Factories, 13, 106.
Ruiz-Villafan,B. Rodriguez-Sanoja,R. Aguilar-Osorio,G. Gosset,G. Sanchez,S.
2014. Glucose kinases from Streptomyces peucetius var. caesius Applied Microbiology and Biotechnology, 98, 6061-6071.
Cortes-Tolalpa,L. Gutierrez-Rios,R.M. Martinez,L.M. de Anda,R. Gosset,G. Bolivar,F. Escalante,A.
2014. Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium Microbial Cell Factories, 13, 28.
Centeno-Leija,S. Huerta-Beristain,G. Giles-Gomez,M. Bolivar,F. Gosset,G. Martinez,A.
2014. Improving poly-3-hydroxybutyrate production in Escherichia coli by combining the increase in the NADPH pool and acetyl-CoA availability Antonie Van Leeuwenhoek, 105, 687-696.
Sabido,A. Sigala,J.C. Hernandez-Chavez,G. Flores,N. Gosset,G. Bolivar,F.
2014. Physiological and transcriptional characterization of Escherichia coli strains lacking interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are coutilized Biotechnology and Bioengineering, 111, 1150-1160.
Licona-Cassani,C. Lara,A.R. Cabrera-Valladares,N. Escalante,A. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G.
2014. Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis Journal of Molecular Microbiology and Biotechnology, 24, 37-45.
Wunderlich,M. Taymaz-Nikerel,H. Gosset,G. Ramirez,O.T. Lara,A.R.
2014. Effect of growth rate on plasmid DNA production and metabolic performance of engineered Escherichia coli strains Journal of Bioscience and Bioengineering, 117, 336-342.
Chavez-Bejar,M.I. Balderas-Hernandez,V.E. Gutierrez-Alejandre,A. Martinez,A. Bolivar,F. Gosset,G.
2013. Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose Microbial Cell Factories, 12, 108.
Rodriguez,A. Martinez,J.A. Baez-Viveros,J.L. Flores,N. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Bolivar,F.
2013. Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF Microbial Cell Factories, 12, 86.
Centeno-Leija,S. Utrilla,J. Flores,N. Rodriguez,A. Gosset,G. Martinez,A.
2013. Metabolic and transcriptional response of Escherichia coli with a NADP-dependent glyceraldehyde 3-phosphate dehydrogenase from Streptococcus mutans Antonie Van Leeuwenhoek, 104, 913-924.
Fuentes,L.G. Lara,A.R. Martinez,L.M. Ramirez,O.T. Martinez,A. Bolivar,F. Gosset,G.
2013. Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production Microbial Cell Factories, 12, 42.
Soria,S. de Anda,R. Flores,N. Romero-Garcia,S. Gosset,G. Bolivar,F. Baez-Viveros,J.L.
2013. New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli Journal of Basic Microbiology, 53, 365-380.
Sabido,A. Martinez,L.M. de Anda,R. Martinez,A. Bolivar,F. Gosset,G.
2013. A novel plasmid vector designed for chromosomal gene integration and expression: Use for developing a genetically stable Escherichia coli melanin production strain Plasmid, 69, 16-23.
Borja,G.M. Meza-Mora E. Barron,B. Gosset,G. Ramirez,O.T. Lara,A.R.
2012. Engineering Escherichia coli to increase plasmid DNA production in high cell-density cultivations in batch mode Microbial Cell Factories, 11, 132.
Meza,E. Becker,J. Bolivar,F. Gosset,G. Wittmann,C.
2012. Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli Microbial Cell Factories, 11, 127.
Utrilla,J. Licona-Cassani,C. Marcellin,E. Gosset,G. Nielsen,L.K. Martinez,A.
2012. Engineering and adaptive evolution of Escherichia coli for d-lactate fermentation reveals GatC as a xylose transporter Metabolic Engineering, 14, 469-476.
Aguilar,C. Escalante,A. Flores,N. de Anda,R. Riveros-McKay,F. Gosset,G. Morett,E. Bolivar,F.
2012. Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system BMC Genomics, 13, 385.
Cabrera-Valladares,N. Martinez,L.M. Flores,N. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G.
2012. Physiologic Consequences of Glucose Transport and Phosphoenolpyruvate Node Modifications in Bacillus subtilis 168 Journal of Molecular Microbiology and Biotechnology, 22, 177-197.
Fernandez-Sandoval,M.T. Huerta-Beristain,G. Trujillo-Martinez,B. Bustos,P. Gonzalez,V. Bolivar,F. Gosset,G. Martinez,A.
2012. Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium Applied Microbiology and Biotechnology, 96, 1291-1300.
Munoz-Gutierrez,I. Oropeza,R. Gosset,G. Martinez,A.
2012. Cell surface display of a beta-glucosidase employing the type V secretion system on ethanologenic Escherichia coli for the fermentation of cellobiose to ethanol Journal of Industrial Microbiology & Biotechnology, 39, 1141-1152.
Chavez-Bejar,M.I. Baez-Viveros,J.L. Martinez,A. Bolivar,F. Gosset,G.
2012. Biotechnological production of l-tyrosine and derived compounds Process Biochemistry, 47, 1017-1026.
Escalante,A. Salinas-Cervantes A. Gosset,G. Bolivar,F.
2012. Current knowledge of the Escherichia coli phosphoenolpyruvate-carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation Applied Microbiology and Biotechnology, 94, 1483-1494.
Martinez-Gomez,K. Flores,N. Castaneda,H.M. Martinez-Batallar,G. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Encarnacion,S. Bolivar,F.
2012. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol Microbial Cell Factories, 11, 46.
Pablos,T.E. Soto,R. Mora,E.M. Le Borgne,S. Ramirez,O.T. Gosset,G. Lara,A.R.
2012. Enhanced production of plasmid DNA by engineered Escherichia coli strains Journal of Biotechnology, 158, 211-214.
Soto,R. Caspeta,L. Barron,B. Gosset,G. Ramirez,O.T. Lara,A.R.
2011. High cell-density cultivation in batch mode for plasmid DNA production by a metabolically engineered E. coli strain with minimized overflow metabolism Biochemical Engineering Journal, 56, 165-171.
Lopes,M.S.G. Gosset,G. Rocha,R.C.S. Gomez,J.G.C. Ferreira da Silva,L.
2011. PHB Biosynthesis in Catabolite Repression Mutant of Burkholderia sacchari Current Microbiology, 63, 319-326.
Pablos,T.E. Mora,E.M. Le Borgne,S. Ramirez,O.T. Gosset,G. Lara,A.R.
2011. Vitreoscilla hemoglobin expression in engineered Escherichia coli: Improved performance in high cell-density batch cultivations Biotechnology Journal, 6, 993-1002.
Munoz,A.J. Hernandez-Chavez,G. de Anda,R. Martinez,A. Bolivar,F. Gosset,G.
2011. Metabolic engineering of Escherichia coli for improving L: -3,4-dihydroxyphenylalanine (L: -DOPA) synthesis from glucose Journal of Industrial Microbiology & Biotechnology, 38, 1845-1852.
Tierrafria,V.H. Ramos-Aboites,H.E. Gosset,G. Barona-Gomez,F.
2011. Disruption of the siderophore-binding desE receptor gene in Streptomyces coelicolor A3(2) results in impaired growth in spite of multiple iron-siderophore transport systems Microbial Biotechnology, 4, 275-285.
Balderas-Hernandez,V.E. Hernandez-Montalvo,V. Bolivar,F. Gosset,G. Martinez,A.
2011. Adaptive Evolution of Escherichia coli Inactivated in the Phosphotransferase System Operon Improves Co-utilization of Xylose and Glucose Under Anaerobic Conditions Applied Biochemistry and Biotechnology, 163, 485-496.
Escalante,A. Calderon,R. Valdivia,A. de Anda,R. Hernandez,G. Ramirez,O.T. Gosset,G. Bolivar,F.
2010. Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Microbial Cell Factories, 9, 21.
Gosset,G.
2009. Production of aromatic compounds in bacteria Current Opinion in Biotechnology, 20, 651-658.
Olvera,L. Mendoza-Vargas,A. Flores,N. Olvera,M. Sigala,J.C. Gosset,G. Morett,E. Bolivar,F.
2009. Transcription Analysis of Central Metabolism Genes in Escherichia coli. Possible Roles of a38 in Their Expression, as a Response to Carbon Limitation PLoS ONE, 4, e7466.
Vazquez,C.D. Freyre-Gonzalez,J.A. Gosset,G. Loza,J.A. Gutierrez-Rios,R.M.
2009. Identification of network topological units coordinating the global expression response to glucose in Bacillus subtilis and its comparison to Escherichia coli BMC Microbiology, 9, 176.
Utrilla,J. Gosset,G. Martinez,A.
2009. ATP limitation in a pyruvate formate lyase mutant of Escherichia coli MG1655 increases glycolytic flux to D: -lactate Journal of Industrial Microbiology & Biotechnology, 36, 1057-1062.
Romero-Garcia,S. Hernandez-Bustos,C. Merino,E. Gosset,G. Martinez,A.
2009. Homolactic fermentation from glucose and cellobiose using Bacillus subtilis Microbial Cell Factories, 8, 23.
Balderas-Hernandez,V.E. Sabido-Ramos,A. Silva,P. Cabrera-Valladares,N. Hernandez-Chavez,G. Baez-Viveros,J.L. Martinez,A. Bolivar,F. Gosset,G.
2009. Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli Microbial Cell Factories, 8, 19.
Sigala,J.C. Flores,S. Flores,N. Aguilar,C. de Anda,R. Gosset,G. Bolivar,F.
2009. Acetate Metabolism in Escherichia coli Strains Lacking Phosphoenolpyruvate: Carbohydrate Phosphotransferase System; Evidence of Carbon Recycling Strategies and Futile Cycles Journal of Molecular Microbiology and Biotechnology, 16, 224-235.
Orencio-Trejo,M. Flores,N. Escalante,A. Hernandez-Chavez,G. Bolivar,F. Gosset,G. Martinez,A.
2008. Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities Biotechnology for Biofuels, 1, 8.
Escalante,A. Giles-Gomez,M. Hernandez,G. Cordova-Aguilar,M.S. Lopez-Munguia,A. Gosset,G. Bolivar,F.
2008. Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach International Journal of Food Microbiology, 124, 126-134.
Huerta-Beristain,G. Utrilla,J. Hernandez-Chavez,G. Bolivar,F. Gosset,G. Martinez,A.
2008. Specific ethanol production rate in ethanologenic Escherichia coli strain KO11 Is limited by pyruvate decarboxylase Journal of Molecular Microbiology and Biotechnology, 15, 55-64.
Chavez-Bejar,M.I. Lara,A.R. Lopez,H. Hernandez-Chavez,G. Martinez,A. Ramirez,O.T. Bolivar,F. Gosset,G.
2008. Metabolic engineering of Escherichia coli for L-tyrosine production by the expression of the genes coding for the chorismate mutase domain from native P-protein and a cyclohexadienyl dehydrogenase from Zymomonas mobilis Applied and Environmental Microbiology, 74, 3284-3290.
Martinez,K. de Anda,R. Hernandez,G. Escalante,A. Gosset,G. Ramirez,O.T. Bolivar,F.G.
2008. Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Microbial Cell Factories, 7, 1.
Flores,N. Escalante,A. de Anda,R. Baez-Viveros,J.L. Merino,E. Franco,B. Georgellis,D. Gosset,G. Bolivar,F.
2008. New Insights into the Role of Sigma Factor RpoS as Revealed in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Journal of Molecular Microbiology and Biotechnology, 14, 176-192.
Lara,A.R. Caspeta,L. Gosset,G. Bolivar,F. Ramirez,O.T.
2008. Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: An alternative to fed-batch cultures Biotechnology and Bioengineering, 99, 893-901.
Vazquez-Limon,C. Vega-Badillo,J. Martinez,A. Espinosa-Molina,G. Gosset,G. Soberon,X. Lopez-Munguia,A. Osuna,J.
2007. Growth rate of a non-fermentative Escherichia coli strain is influenced by NAD(+) regeneration Biotechnology Letters, 29, 1857-1863.
Baez-Viveros,J.L. Flores,N. Suarez,K. Castillo-Espana,P. Bolivar,F. Gosset,G.
2007. Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine Microbial Cell Factories, 6, 30.
Pinero,S. Rivera,J. Romero,D. Cevallos,M.A. Martinez,A. Bolivar,F. Gosset,G.
2007. Tyrosinase from Rhizobium etli Is Involved in Nodulation Efficiency and Symbiosis-Associated Stress Resistance Journal of Molecular Microbiology and Biotechnology, 13, 35-44.
Flores,N. Leal,L. Sigala,J.C. de Anda,R. Escalante,A. Martinez,A. Ramirez,O.T. Gosset,G. Bolivar,F.
2007. Growth Recovery on Glucose under Aerobic Conditions of an Escherichia coli Strain Carrying a Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Deletion by Inactivating arcA and Overexpressing the Genes Coding for Glucokinase and Galactose Permease Journal of Molecular Microbiology and Biotechnology, 13, 105-116.
Trevino-Quintanilla,L.G. Escalante,A. Caro,A.D. Martinez,A. (error para richar) Puente,J.L. Bolivar,F. Gosset,G.
2007. The Phosphotransferase System-Dependent Sucrose Utilization Regulon in Enteropathogenic Escherichia coli Strains Is Located in a Variable Chromosomal Region Containing iap Sequences Journal of Molecular Microbiology and Biotechnology, 13, 117-125.
Romero,S. Merino,E. Bolivar,F. Gosset,G. Martinez,A.
2007. Metabolic engineering of Bacillus subtilis for ethanol production: Lactate dehydrogenase plays a key role in the fermentative metabolism Applied and Environmental Microbiology, 73, 5190-5198.
Gutierrez-Rios,R.M. Freyre-Gonzalez,J.A. Resendis,O. Collado-Vides,J. Saier,M. Gosset,G.
2007. Identification of regulatory network topological units coordinating the genome-wide transcriptional response to glucose in Escherichia coli BMC Microbiology, 7, 53.
Lagunas-Munoz,V.H. Cabrera-Valladares,N. Bolivar,F. Gosset,G. Martinez,A.
2006. Optimum melanin production using recombinant Escherichia coli Journal of Applied Microbiology, 101, 1002-1008.
Lara,A.R. Vazquez-Limon,C. Gosset,G. Bolivar,F. Lopez-Mungua,A. Ramirez,O.T.
2006. Engineering Escherichia coli to improve culture performance and reduce formation of by-products during recombinant protein production under transient intermittent anaerobic conditions Biotechnology and Bioengineering, 94, 1164-1175.
Cabrera-Valladares,N. Martinez,A. Pinero,S. Lagunas-Munoz,V.H. Tinoco,R. de Anda,R. Vazquez-Duhalt,R. Bolivar,F. Gosset,G.
2006. Expression of the melA gene from Rhizobium etli CFN42 in Escherichia coli and characterization of the encoded tyrosinase Enzyme and Microbial Technology, 38, 772-779.
de Anda,R. Lara,A.R. Hernandez,V. Hernandez-Montalvo,V. Gosset,G. Bolivar,F. Ramirez,O.T.
2006. Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate Metabolic Engineering, 8, 281-290.
Lara,A.R. Leal,L. Flores,N. Gosset,G. Bolivar,F. Ramirez,O.T.
2006. Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system Biotechnology and Bioengineering, 93, 372-385.
Flores,S. Flores,N. de Anda,R. Gonzalez,A. Escalante,A. Sigala,J.C. Gosset,G. Bolivar,F.
2005. Nutrient-Scavenging Stress Response in an Escherichia coli Strain Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System, as Explored by Gene Expression Profile Analysis Journal of Molecular Microbiology and Biotechnology, 10, 51-63.
Gosset,G.
2005. Improvement of Escherichia coli production strains by modification of the phosphoenolpyruvate:sugar phosphotransferase system Microbial Cell Factories, 4, 14.
Flores,N. Flores,S. Escalante,A. de Anda,R. Leal,L. Malpica,R. Georgellis,D. Gosset,G. Bolivar,F.
2005. Adaptation for fast growth on glucose by differential expression of central carbon metabolism and gal regulon genes in an Escherichia coli strain lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system Metabolic Engineering, 7, 70-87.
Zhang,Z. Gosset,G. Barabote,R. Gonzalez,C.S. Cuevas,W.A. Saier,M.H., Jr.
2005. Functional Interactions between the Carbon and Iron Utilization Regulators, Crp and Fur, in Escherichia coli Journal of Bacteriology, 187, 980-990.
Sandoval-Basurto,E.A. Gosset,G. Bolivar,F. Ramirez,O.T.
2005. Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: Metabolic response and production of recombinant protein Biotechnology and Bioengineering, 89, 453-463.
Flores,N. de Anda,R. Flores,S. Escalante,A. Hernandez,G. Martinez,A. Ramirez,O.T. Gosset,G. Bolivar,F.
2004. Role of Pyruvate Oxidase in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Journal of Molecular Microbiology and Biotechnology, 8, 209-221.
Flores,S. Anda-Herrera,R. Gosset,G. Bolivar,F.G.
2004. Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway Biotechnology and Bioengineering, 87, 485-494.
Baez-Viveros,J.L. Osuna,J. Hernandez-Chavez,G. Soberon,X. Bolivar,F. Gosset,G.
2004. Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli Biotechnology and Bioengineering, 87, 516-524.
Escalante,A. Rodriguez,M.E. Martinez,A. Lopez-Munguia,A. Bolivar,F. Gosset,G.
2004. Characterization of bacterial diversity in Pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis Fems Microbiology Letters, 235, 273-279.
Gosset,G. Zhang,Z. Nayyar,S. Cuevas,W.A. Saier,M.H., Jr.
2004. Transcriptome Analysis of Crp-Dependent Catabolite Control of Gene Expression in Escherichia coli Journal of Bacteriology, 186, 3516-3524.
Garay-Arroyo,A. Covarrubias,A.A. Clark,I. Nino,I. Gosset,G. Martinez,A.
2004. Response to different environmental stress conditions of industrial and laboratory Saccharomyces cerevisiae strains Applied Microbiology and Biotechnology, 63, 734-741.
Hernandez-Montalvo,V. Martinez,A. Bolivar,F. Valle,F. Gosset,G.
2003. Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products Biotechnology and Bioengineering, 83, 687-694.
Flores,S. Gosset,G. Flores,N. de Graaf,A.A. Bolivar,F.
2002. Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by 13C labelling and NMR spectroscopy Metabolic Engineering, 4, 124-137.
Balbas,P. Gosset,G.
2001. Chromosomal editing in Escherichia coli. Vectors for DNA integration and excision Molecular Biotechnology, 19, 1-12.
Hernandez-Montalvo,V. Valle,F. Bolivar,F. Gosset,G.
2001. Characterization of sugar mixtures utilization by an Escherichia coli mutant devoid of the phosphotransferase system Applied Microbiology and Biotechnology, 57, 186-191.
Le Borgne,S. Palmeros,B. Bolivar,F. Gosset,G.
2001. Improvement of the pBRINT-Ts plasmid family to obtain marker-free chromosomal insertion of cloned DNA in E. coli Biotechniques, 30, 252-256.
Baez,J.L. Bolivar,F. Gosset,G.
2001. Determination of 3-deoxy-D-arabino-heptulosonate 7-phosphate productivity and yield from glucose in Escherichia coli devoid of the glucose phosphotransferase transport system Biotechnology and Bioengineering, 73, 530-535.
Gosset,G. Bonner,C.A. Jensen,R.A.
2001. Microbial Origin of Plant-Type 2-Keto-3-Deoxy-D-arabino-Heptulosonate 7-Phosphate Synthases, Exemplified by the Chorismate- and Tryptophan-Regulated Enzyme from Xanthomonas campestris Journal of Bacteriology, 183, 4061-4070.
Palmeros,B. Wild,J. Szybalski,W. Le Borgne,S. Hernandez-Chavez,G. Gosset,G. Valle,F. Bolivar,F.
2000. A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria Gene, 247, 255-264.
Cruz,N. Le Borgne,S. Hernandez-Chavez,G. Gosset,G. Valle,F. Bolivar,F.
2000. Engineering the Escherichia coli outer membrane protein OmpC for metal bioadsorption Biotechnology Letters, 22, 623-629.
Le Borgne,S. Palmeros,B. Valle,F. Bolivar,F. Gosset,G.
1998. pBRINT-Ts: a plasmid family with a temperature-sensitive replicon, designed for chromosomal integration into the lacZ gene of Escherichia coli Gene, 223, 213-219.
Gosset,G. Yong-Xiao,J. Berry,A.
1996. A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli Journal of Industrial Microbiology, 17, 47-52.
Pfister,C.U. Duval,M. Godbillon,J. Gosset,G. Gygax,D. Marfil,F. Sioufi,A. Winkler,B.
1994. Development, application and comparison of an enzyme immunoassay and a high-performance liquid chromatography method for the determination of the aromatase inhibitor CGS 20,267 in biological fluids Journal of Pharmaceutical Sciences, 83, 520-524 * .
Olmos,J. Cruz,N. Sanchez,M. Lopez,M. Balbas,P. Gosset,G. Valle,F. Bolivar,F.
1994. Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography Journal of Biotechnology, 38, 89-96.
Gosset,G. de Anda,R. Cruz,N. Martinez,A. Quintero,R. Bolivar,F.
1993. Recombinant protein production in cultures of an Escherichia coli trp- strain Applied Microbiology and Biotechnology, 39, 541-546.
Cruz,N. Lopez,M. Estrada,G. Alvarado,X. de Anda,R. Balbas,P. Gosset,G. Bolivar,F.
1992. Preparative isolation of recombinant human insulin-A chain by ion exchange chromatography Journal Of Liquid Chromatography, 15, 2311-2324.
Cruz,N. Antonio,S. de Anda,R. Gosset,G. Bolivar,F.
1990. Preparative isolation by high performance liquid chromatography of human insulin B chain produced in Escherichia coli Journal Of Liquid Chromatography, 13, 1517-1528.
Puente,J.L. Alvarez-Scherer,V. Gosset,G. Calva,E.
1989. Comparative analysis of the Salmonella typhi and Escherichia coli ompC genes Gene, 83, 197-206.
Gosset,G. Merino,E. Recillas,F. Oliver,G. Becerril,B. Bolivar,F.
1989. Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12.Protein sequences & data analysis, 2, 9-16.
Oliver,G. Gosset,G. Sanchez-Pescador,R. Lozoya,E. Ku,L.M. Flores,N. Becerril,B. Valle,F. Bolivar,F.
1987. Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12 Gene, 60, 1-11.
Valle,F. Gosset,G. Tenorio,B. Oliver,G. Bolivar,F.
1986. Characterization of the regulatory region of the Escherichia coli penicillin acylase structural gene Gene, 50, 119-122.
Oliver,G. Valle,F. Rosetti,F. Gomez-Pedrozo,M. Santamaria,P. Gosset,G. Bolivar,F.
1985. A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105 Gene, 40, 9-14.
Libros y capítulos recientes Lara,A.R. Gosset,G. 2020. Minimal Cells: Design, Construction, Biotechnological Applications Cham:. Springer.
Gosset-Lagarda,G. 2018. Generación de una cepa de Escherichia coli para la producción de L-tirosina en: Quintero-Ramirez,R. Problemas de ingeniería biológica. Mexico. Universidad Autónoma Metropolitana, Unidad Cuajimalpa. pags. 387-394
Gosset,G. 2017. Biotechnological Production of Melanins with Microorganisms en: O.V.Singh Bio-pigmentation and Biotechnological Implementations. John Wiley & Sons, Inc.. pags. 161-171
Martinez,A. Rodriguez-Alegria,M. Fernandes,M.C. Gosset,G. Vargas-Tah,A. 2017. Metabolic Engineering of Escherichia coli for Lactic Acid Production from Renewable Resources en: *gosset Engineering of Microorganisms for the Production of Chemicals and Biofuels from Renewable Resources. Cham. Springer International Publishing. pags. 125-145
Gosset,G. 2017. Engineering of Microorganisms for the Production of Chemicals and Biofuels from Renewable Resources Cham:. Springer.
Escalante-Lozada,A. Giles-Gomez,M. Gosset-Lagarda,G. 2014. El pulque, una bebida histórica con importantes implicaciones biotecnológicas en: Rebolledo,F. Un Mundo de Bacterias. UNAM, Campus Morelos. pags. 91-95
Orencio-Trejo,M. Utrilla,J. Fernandez-Sandoval,M.T. Huerta-Beristain,G. Gosset,G. Martinez,A. 2010. Engineering the Escherichia coli Fermentative Metabolism en: Krull,R. Biosystems Engineering II. Springer. pags. 71-107
Escalante,A. Martinez,A. Rivera,M. Gosset,G. 2009. Section 1 en: Smolke,C.D. Metabolic Pathway Engineering Handbook. CRC Press. pags. 10-20
Martinez-Jimenez,A. Gosset-Lagarda,G. 2008. Ingeniería metabólica de bacterias, cap 32 en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario. Mexico, D.F.. UNAM. pags. 373-384
Chavez-Bejar,M. Martinez,A. Bolivar,F. Gosset,G. 2005. Metabolic pathway engineering for microbial production of aromatic amino acids en: Moham,R.M. Research Advances in Food Science. Kerala. Global Research network. pags. 11-20
Le Borgne,S. Bolivar,F. Gosset,G. 2004. Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli en: Lorence,A. Recombinant gene expression : reviews and protocols. pags. 135-144
Gosset,G. Bolivar,F. 2004. Ingenieria celular microbiana en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 219-247
Gosset,G. Bolivar,F. 2003. Ingeniería celular en microorganismos en: *agustin Fronteras de la Biología en los Inicios del Siglo XXI. Módulo VII. Mexico,D.F:. El Colegio Nacional, México. pags. 5-34
Gosset,G. Bolivar,F. Soberon,X. Lopez-Munguia,A. Valderrama,B. Ayala,M. Vazquez,R. Ramirez,O.T. Alagon,A. Possani,L.D. 2003. Fronteras de la Biología en los Inicios del Siglo XXI. Módulo VII: Ingeniería Celular, Biodiversidad e Industria. Mexico, D.F.: El Colegio Nacional.
LaDuca,R.J. Berry,A. Chotani,G. Dodge,T. Gosset,G. Valle,F. Liao,J. Xiao,J. Power,S. 1999. Metabolic Pathway Engineering of Aromatic Compounds en: Manual of industrial microbiology and biotechnology. Washington, D.C. ASM Press. pags. 605-615
Otras Publicaciones Velazquez,D. Gosset,G. Lara,A.R.
2020. Ingeniería Celular como una Alternativa al Cultivo por lote Alimentado para la Producción de Proteínas Recombinantes Biotecnologia, 24, 30-55.
Sassi,H. Nguyen,T.M. Telek,S. Gosset,G. Grunberger,A. Delvigne,F.
2019. Segregostat: A novel concept to control phenotypic diversification dynamics on the example of Gram-negative bacteria bioRxiv, Preprint posted February 08, 2019., 542704.
Gosset,G.
2018. Los microbios como biofábricas.Selecciones, Marzo, 78-86.
Gosset,G.
2017. Los microbios como biofábricas Ciencia, Revista de la Academia Mexicana de Ciencia, 68, 44-49.
Sabido,A. Martinez,A. Bolivar,F. Gosset,G.
2011. Generación de un Sistema para la Integración y Expresión de Genes en el Cromosoma de Escherichia coli Biotecnologia, 15, 35-49.
Cortes-Tolalpa,L. Carmona,S.B. Cervantes-Salinas,A. Garcia,M.A. Gosset,G. Escalante,A. Bolivar,F.
2011. Ingeniería de vías metabólicas en Escherichia coli para la producción de Shikimato como precursos para la síntesis de compuestos antivirales contra influenza. Biotecnologia, 15, 30-47.
Gosset-Lagarda,G.
2009. La melanina: producción biotecnológica y aplicaciones Hypatia, 29, .
Escalante,A. Valdivia,A. Gosset,G. Bolivar,F.
2009. Ingeniería de Vías Metabólicas en Escherichia Coli Pts– para la Obtención de Cepas Sobreproductoras de Shikimato Mensaje Bioquimico, 33, 171-180.
Romero,S. Orencio,M. Martinez,A. Gosset,G.
2008. Compuestos aromáticos e ingeniería metabólica en bacterias Ciencia y desarrollo, 34, 8-13.
Gosset,G.
2008. Microbial production of industrial chemicals. Introduction Journal of Molecular Microbiology and Biotechnology, 15, 5-7.
Le Borgne,S. Gosset,G.
2006. Sharing microbiology and oil biotechnology experiences Revista Latinoamericana de Microbiologia, 48, 95-98.
Baez-Viveros,J.L. Osuna,J. Hernandez-Chavez,G. Soberon,X. Bolivar,F. Gosset,G.
2006. Metabolic via engineering for the production of aromatic substances in Escherichia coli Revista Latinoamericana de Microbiologia, 48, 92.
Martinez,A. Rodriguez,M.E. Lopez-Munguia,A. Gosset,G.
2006. ¿Etanol carburante a base de bagazo de caña? Claridades Agropecuarias, 155, 22-25.
Lagarda,G.G.
2006. [Industrial microbiology] Revista Latinoamericana de Microbiologia, 48, 91-98 * .
Escalante-Lozada,A. Gosset-Lagarda,G. Martinez-Jimenez,A. Bolivar-Zapata,F.
2004. Soil bacterial diversity: Microbial culture-independent methods of study and biotechnological implications Agrociencia, 38, 583-592.
Martinez,A. Bolivar,F. Gosset,G.
2002. Biotecnologia energetica sustentable: etanol carburante para el transporte Revista Universidad de Mexico, 617, .
Patentes José Manuel Camacho Zaragoza, Georgina Teresa Hernández Chávez, Fabián Moreno Avitia, René Ramírez Iñiguez, Alfredo Martínez Jiménez , Francisco Bolívar Zapata, Guillermo Gosset Lagarda 2017 Cepas de Escherichia coli modificadas por ingeniería de vías metabólicas para la biosíntesis de resveratrol y su uso en un nuevo proceso de co-cultivo.UNAM México. (en trámite)
Alfredo Martínez Jiménez, Guillermo Gosset Lagarda, Luz María Martínez Mejía, Georgina Teresa Hernández Chávez, Mauricio García Benítez 2016 Cepas de Escherichia coli modificadas por ingeniería metabólica para la producción de (R)-3-hidroxibutirato.UNAM México. (en trámite)
A. Martínez J. G. Gosset L. G. T. Hernández Ch. G. Huerta B. B. Trujillo y M., J. Utrilla C. 2013 Strains of Escherichia coli modified by metabolic engineering to produce chemical compounds from hydrolyzed lignocellulose, pentoses, hexoses or other carbon sources.UNAM EUA 8563283, México No. 31474 y 3127021.
Ponce Laura Grecia , Martínez Mejía Luz María , Ramírez Reivich Octavio Tonatiuh , Martínez Jiménez Alfredo , Bolívar Zapata Francisco Gonzalo , Gosset Lagarda Guillermo . 2013 Cepas de Escherichia coli con alta capacidad para la producción de plásmidos y su uso en la producción de vacunas de ADN.UNAM México. (en trámite)
Guillermo Gosset Lagarda , Alfredo Martínez Jiménez , Francisco Gonzalo Bolívar Zapata , Ana Joyce Muñoz Arellano, Georgina Teresa Hernández Chávez y Ramón de Anda Herrera 2011 Cepas de Escherichia coli modificadas para producir 3,4-hidroxi-L-fenilalanina (L-Dopa).UNAM México. (en trámite)
José Utrilla Carreri, Luz María Martínez Mejía , Guillermo Gosset Lagarda , y Alfredo Martínez Jiménez 2011 Nuevos transportadores de xilosa y sus aplicaciones. UNAM México No. 340987.
A. R. Lara R., M. de C. Vázquez L., G. Gosset L., F. G. Bolívar Z., A. Lopez-Munguia C. y O. T. Ramírez R 2006 Estrategia para generar células insensibles a condiciones heterogéneas en biorreactores industriales a través de mutaciones de vías metabólicas anaerobias.UNAM México. (en trámite)
J. Osuna, J.L. Báez, G. Hernández, F. G. Bolivar, X. Soberón y G. Gosset 2005 Versiones insensibles a inhibición alostérica y catalíticamente eficientes de la enzima corismato mutasa-prefenato deshidratasa y su aplicación para la producción de L-fenilalanina en microorganismos.
G. Gosset, A. Martínez, F. G. Bolívar, V. H. Lagunas, N. Cabrera, J. G. Dávila, V. M. González y V. P. Bustos 2004 Producción de melaninas en microorganismos recombinantes.UNAM México. (en trámite)
F. G. Bolívar Z. G. Gosset L. R. de Anda R. Quintero R. A. Martínez F. Valle N. Flores M. 1994 Proceso fermentativo para obtener proteínas híbridas a partir de cepas de Escherichia coli .UNAM México.
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