. Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Jean-Louis Joseph Marie Charli Casalonga

Dr. Jean-Louis Joseph Marie Charli Casalonga

Jefe del Departamento Genética del Desarrollo y Fisiología Molecular
Líder Académico »» Grupo
Investigador
Tutor de Maestría y Doctorado
Nivel III del SNI
Líneas de investigación

email: charli @ibt.unam.mx
Teléfonos
Oficina +52 (777) 329-1633
desde la CDMX 562-27633
red UNAM 27633
Fax +52 (777) 3170805

Laboratorio +52 (777) 329-1620
desde la CDMX 562-27620
red UNAM 27620

- Licenciatura: Matematicas Superiores y Especiales, Liceo Paul Varely (1971) y Química-Biología, Universidad de París VII, Francia (1973)
- Maestría: en Ciencias Fisiologicas y Biología, Universidad de París XII, Francia (1975)
- Doctorado: en Ciencias Naturales (Biofísica), Universidad de París VI, Francia (1978)
- Estancia de Investigación: Laboratorio del Dres. J.F. McKelvy y L. Hersh, de la Universidad de Texas, Centro de Ciencias de la Salud, Departamento de Bioquímica, Dallas, Texas, E.U.A. (1978-1979)
- Estancia de Investigación: Laboratorio del Dr. H. Boyler, de la Universidad de California, Departamento de Bioquímica y Biofísica, en San Francisco, CA, E.U.A. (1979-1980)

Premio de la Academia de la Investigación Científica en el área de Ciencias Naturales (1990)

Estudiantes

M.C. Adair Jonathan Rodriguez

M.C. Ana Elena Castro

Yuvizel Mariela Rivera

Lic. Haydn Esau Urbina

M.C. Karla Yamili Vargas

Publicaciones recientes

Lazcano,I. Rodriguez-Rodriguez,A. Uribe,R.M. Orozco,A. Joseph-Bravo,P. Charli,J.L. 2021. Evolution of thyrotropin-releasing factor extracellular communication units General and Comparative Endocrinology, 305, 113642.

Castillo-Campos,A. Gutierrez-Mata,A. Charli,J.L. Joseph-Bravo,P. 2021. Chronic stress inhibits hypothalamus-pituitary-thyroid axis and brown adipose tissue responses to acute cold exposure in male rats Journal of Endocrinological Investigation, 44, 713-723.

Pascual-Alonso,I. Arrebola-Sanchez,Y. Almeida,F. Valdes-Tresanco,M.E. Rivera-Mendez,L. Hernandez-Zanuy,A. Chappe-Pacheco,M. Sanchez,B. Charli,J.L. 2020. Marine and costal organisms: a source of biomedically relevant dipeptidyl peptidase IV inhibitors Revista Cubana de Ciencias Biológicas, 8, 1-16.

Pascual-Alonso,I. Rivera-Mendez,L. Almeida,F. Valdes-Tresanco,M.E. Arrebola-Sanchez,Y. Hernandez-Zanuy,A. Alvarez-Lajonchere,L. Diaz,D. Sanchez,B. Florent,I. Schmitt,M. Cisneros,M. Charli,J.L. 2020. Marine organisms: a source of biomedically relevant M1, M2 and M17 exopeptidase inhibitors Revista Cubana de Ciencias Biológicas, 8, 1-36.

Rivera-Mendez,L. Arrebola,Y. Valdes-Tresanco,M.E. Diaz-Guevara,L. Bergado,G. Sanchez,B. Charli,J.L. Pascual-Alonso,I. 2020. Bestatin and bacitracin inhibit porcine kidney cortex dipeptidyl peptidase IV activity and reduce human melanoma MeWo cell viability International Journal of Biological Macromolecules, 164, 2944-2952.

Pascual-Alonso,I. Rivera-Mendez,L. Valdes-Tresanco,M.E. Bounaadja,L. Schmitt.M. Arrebola-Sanchez,Y. Alvarez-Lajonchere,L. Charli,J.L. Florent,I. 2020. Biochemical evidences for M1-, M17- and M18-like aminopeptidases in marine invertebrates from Cuban coastline Zeitschrift für Naturforschung C, 75, 397-407.

Charli,J.L. Rodriguez-Rodriguez,A. Hernandez-Ortega,K. Cote-Velez,A. Uribe,R.M. Jaimes-Hoy,L. Joseph-Bravo,P. 2020. The Thyrotropin-Releasing Hormone-Degrading Ectoenzyme, a Therapeutic Target? Frontiers in Pharmacology, 11, 640.

Joseph-Bravo,P. Lazcano,I. Jaimes-Hoy,L. Gutierrez-Mariscal,M. Sanchez-Jaramillo,E. Uribe,R.M. Charli,J.L. 2020. Sexually dimorphic dynamics of thyroid axis activity during fasting in rats Frontiers in Bioscience, Landmark, 25, 1305-1323.

Farkas,E. Varga,E. Kovacs,B. Szilvasy-Szabo,A. Cote-Velez,A. Peterfi,Z. Matziari,M. Toth,M. Zelena,D. Mezriczky,Z. Kadar,A. Kovari,D. Watanabe,M. Kano,M. Mackie,K. Rozsa,B. Ruska,Y. Toth,B. Mate,Z. Erdelyi,F. Szabo,G. Gereben,B. Lechan,R.M. Charli,J.L. Joseph-Bravo,P. Fekete,C. 2020. A glial-neuronal circuit in the median eminence regulates thyrotropin-releasing hormone-release via the endocannabinoid system iScience, 23, 100921.

Pascual-Alonso,I. Alonso-Bosch,R. Cabrera-Munoz,A. Perera,W. Charli,J.L. 2019. Methanolic extracts of paratoid gland secretion from Cuban Peltophryne toads contain inhibitory activities against peptidases with biomedical relevance Biotecnologia Aplicada, 36, 2221-2227.

Arrebola,Y. Rivera,L. Pedroso,A. McGuire,R. Tresanco,M.E.V. Bergado,G. Charli,J.L. Sanchez,B. Pascual-Alonso,I. 2019. Bacitracin is a non-competitive inhibitor of porcine M1 family neutral and glutamyl aminopeptidases Natural Product Research, Oct 25 [Epub ahead of print], .

Jaimes-Hoy,L. Romero,F. Charli,J.L. Joseph-Bravo,P. 2019. Sex Dimorphic Responses of the Hypothalamus-Pituitary-Thyroid Axis to Maternal Separation and Palatable Diet Frontiers in Endocrinology, 10, 445.

Rodriguez-Rodriguez,A. Lazcano,I. Sanchez-Jaramillo,E. Uribe,R.M. Jaimes-Hoy,L. Joseph-Bravo,P. Charli,J.L. 2019. Tanycytes and the Control of Thyrotropin-Releasing Hormone Flux Into Portal Capillaries Frontiers in Endocrinology, 10, 401.

Parra-Montes de Oca,M.A. Gutierrez-Mariscal,M. Salmeron-Jimenez,M.F. Jaimes-Hoy,L. Charli,J.L. Joseph-Bravo,P. 2019. Voluntary Exercise-Induced Activation of Thyroid Axis and Reduction of White Fat Depots Is Attenuated by Chronic Stress in a Sex Dimorphic Pattern in Adult Rats Frontiers in Endocrinology, 10, 418.

Pascual,I. Valiente,P.A. Garcia,G. Valdes-Tresanco,M.E. Arrebola,Y. Diaz,L. Bounaadja,L. Uribe,R.M. Pacheco,M.C. Florent,I. Charli,J.L. 2017. Discovery of novel non-competitive inhibitors of mammalian neutral M1 aminopeptidase (APN) Biochimie, 142, 216-225.

Jaimes-Hoy,L. Gurrola,G.B. Cisneros,M. Joseph-Bravo,P. Possani,L.D. Charli,J.L. 2017. The Kv1.3 channel blocker Vm24 enhances muscle glucose transporter 4 mobilization but does not reduce body-weight gain in diet-induced obese male rats Life Sciences, 181, 23-30.

Cote-Velez,A. Martinez-Baez A. Lezama,L. Uribe,R.M. Joseph-Bravo,P. Charli,J.L. 2017. A screen for modulators reveals that orexin-A rapidly stimulates thyrotropin releasing hormone expression and release in hypothalamic cell culture Neuropeptides, 62, 11-20.

Sotelo-Rivera,I. Cote-Velez,A. Uribe,R.M. Charli,J.L. Joseph-Bravo,P. 2017. Glucocorticoids curtail stimuli-induced CREB phosphorylation in TRH neurons through interaction of the glucocorticoid receptor with the catalytic subunit of protein kinase A Endocrine, 55, 861-871.

Joseph-Bravo,P. Jaimes-Hoy,L. Charli,J.L. 2016. Advances in TRH signaling Reviews in Endocrine and Metabolic Disorders, 17, 545-558.

Jaimes-Hoy,L. Gutierrez-Mariscal,M. Vargas,Y. Perez-Maldonado,A. Romero,F. Sanchez-Jaramillo,E. Charli,J.L. Joseph-Bravo,P. 2016. Neonatal maternal separation alters, in a sex specific manner, the expression of TRH, of TRH-degrading ectoenzyme in the rat hypothalamus, and the response of the thyroid axis to starvation Endocrinology, 157, 3253-3265.

Joseph-Bravo,P. Jaimes-Hoy,L. Uribe,R.M. Charli,J.L. 2015. TRH, first hypophysiotropic releasing hormone isolated: its control of pituitary-thyroid axis Journal of Endocrinology, 226, T85-T100 [erratum en vol 227 (3) X3].

Lazcano,I. Cabral,A. Uribe,R.M. Jaimes-Hoy,L. Perello,M. Joseph-Bravo,P. Sanchez-Jaramillo,E. Charli,J.L. 2015. Fasting enhances pyroglutamyl peptidase II activity in tanycytes of the mediobasal hypothalamus of male adult rats Endocrinology, 156, 2713-2723.

Joseph-Bravo,P. Jaimes-Hoy,L. Charli,J. 2015. Regulation of TRH neurons and energy homeostasis related signals under stress Journal of Endocrinology, 224, R139-R159 [erratum en vol 227 (3) X1].

Martinez-Armenta,M. de Leon-Guerrero,S.D. Catalan,A. Alvarez-Arellano,L. Uribe,R.M. Subramaniam,M. Charli,J.L. Perez-Martinez,L. 2015. TGFbeta2 regulates hypothalamic Trh expression through the TGFbeta inducible early gene-1 (TIEG1) during fetal development Molecular and Cellular Endocrinology, 400, 129-139.

Gonzalez-Bacerio,J. Fando,R. Monte-Martinez,A.D. Charli,J.L. Chavez,M.D. 2014. Plasmodium falciparum M1-Aminopeptidase: a Promising Target for the Development of Antimalarials Current Drug Targets, 15, 1144-1165.

Sotelo-Rivera,I. Jaimes-Hoy,L. Cote-Velez,A. Espinoza-Ayala,C. Charli,J.L. Joseph-Bravo,P. 2014. An acute injection of corticosterone increases thyrotrophin releasing hormone expression in the paraventricular nucleus of the hypothalamus but interferes with the rapid hypothalamus pituitary thyroid axis response to cold in male rats Journal of Neuroendocrinology, 26, 861-869.

Gonzalez-Bacerio,J. Osuna,J. Ponce,A. Fando,R. Figarella,K. Mendez,Y. Charli,J.L. Chavez,M.D. 2014. High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase Protein Expression and Purification, 104, 103-114.

Mendez,Y. Perez-Labrada,K. Gonzalez-Bacerio,J. Valdes,G. Chavez,M.D.A. Osuna,J. Charli,J.L. Pascual,I. Rivera,D.G. 2014. Combinatorial Multicomponent Access to Natural-Products-Inspired Peptidomimetics: Discovery of Selective Inhibitors of Microbial Metallo-aminopeptidases ChemMedChem, 9, 2351-2359.

Rodriguez-Molina,V. Patino,J. Vargas,Y. Sanchez-Jaramillo,E. Joseph-Bravo,P. Charli,J.L. 2014. TRH regulates action potential shape in cerebral cortex pyramidal neurons Brain Research, 1571, 1-11.

Uribe,R.M. Jaimes-Hoy,L. Ramirez-Martinez,C. Garcia-Vazquez,A. Romero,F. Cisneros,M. Cote-Velez,A. Charli,J.L. Joseph-Bravo,P. 2014. Voluntary exercise adapts the hypothalamus-pituitary-thyroid axis in male rats Endocrinology, 155, en20131724.

Gomez,H. Chappe,M. Valiente,P.A. Pons,T. Chavez,M.D.A. Charli,J.L. Pascual,I. 2013. Effect of zinc and calcium ions on the rat kidney membrane-bound form of dipeptidyl peptidase IV Journal of Biosciences, 38, 461-469.

Gutierrez-Mariscal,M. Sanchez,E. Garcia-Vazquez,A. Rebolledo-Solleiro,D. Charli,J.L. Joseph-Bravo,P. 2012. Acute response of hypophysiotropic thyrotropin releasing hormone neurons and thyrotropin Regulatory Peptides, 179, 61-70.

Lazcano,I. Uribe,R.M. Martinez-Chavez,E. Vargas,M.A. Matziari,M. Joseph-Bravo,P. Charli,J.L. 2012. Pyroglutamyl peptidase II inhibition enhances the analeptic effect of thyrotropin releasing hormone in the rat medial septum Journal of Pharmacology and Experimental Therapeutics, 342, 222-231.

Gutierrez-Mariscal,M. Sanchez,E. Rebolledo-Solleiro,D. Garcia-Vazquez,A.I. Cote-Velez,A. Acasuso-Rivero,C. Charli,J.L. Joseph-Bravo,P. 2012. The acute response of the amygdalar TRH system to psychogenic stressors varies dependent on the paradigm and circadian condition Brain Research, 1452, 73-84.

Guerra-Crespo,M. Perez-Monter,C. Janga,S.C. Castillo-Ramirez,S. Gutierrez-Rios,R.M. Joseph-Bravo,P. Perez-Martinez,L. Charli,J.L. 2011. Transcriptional profiling of fetal hypothalamic TRH neurons BMC Genomics, 12, 222.

Cote-Velez,A. Perez-Maldonado,A. Osuna,J. Barrera,B. Charli,J.L. Joseph-Bravo,P. 2011. Creb and Sp/Kruppel response elements cooperate to control rat TRH gene transcription in response to cAMP Biochimica et Biophysica Acta (BBA), 1809, 191-199.

Perez-Monter,C. Martinez-Armenta,M. Miquelajauregui,A. Furlan-Magaril,M. Varela-Echavarria,A. Recillas-Targa,F. May,V. Charli,J.L. Perez-Martinez,L. 2011. The Kruppel-like factor 4 controls biosynthesis of thyrotropin-releasing hormone during hypothalamus development Molecular and Cellular Endocrinology, 333, 127-133.

Pascual,I. Gomez,H. Pons,T. Chappe,M. Vargas,M.A. Valdes,G. Lopez,A. Saroyan,A. Charli,J.L. Chavez,M.D. 2011. Effect of divalent cations on the porcine kidney cortex membrane-bound form of dipeptidyl peptidase IV International Journal of Biochemistry and Cell Biology, 43, 363-371.

Uribe,R.M. Cisneros,M. Vargas,M.A. Lezama,L. Cote-Velez,A. Joseph-Bravo,P. Charli,J.L. 2011. The systemic inhibition of nitric oxide production rapidly regulates TRH mRNA concentration in the paraventricular nucleus of the hypothalamus and serum TSH concentration. Studies in control and cold-stressed rats Brain Research, 1367, 188-197.

Diaz-Gallardo,M.Y. Cote-Velez,A. Charli,J.L. Joseph-Bravo,P. 2010. A rapid interference between glucocorticoids and cAMP-activated signaling in hypothalamic neurons prevents binding of pCREB and GR at the CRE-like and composite GRE sites of TRH gene promoter Journal of Neuroendocrinology, 22, 282-293.

Diaz-Gallardo,M.Y. Cote-Velez,A. Carreon-Rodriguez,A. Charli,J.L. Joseph-Bravo,P. 2010. Phosphorylated Cyclic-AMP-Response Element-Binding Protein and Thyroid Hormone Receptor Have Independent Response Elements in the Rat Thyrotropin-Releasing Hormone Promoter: An Analysis in Hypothalamic Cells Neuroendocrinology, 91, 64-76.

Sanchez,E. Vargas,M.A. Singru,P.S. Pascual,I. Romero,F. Fekete,C. Charli,J.L. Lechan,R.M. 2009. Tanycyte Pyroglutamyl Peptidase II Contributes to Regulation of the Hypothalamic-Pituitary-Thyroid Axis through Glial-Axonal Associations in the Median Eminence Endocrinology, 150, 2283-2291.

Carreon-Rodriguez,A. Charli,J.L. Perez-Martinez,L. 2009. T3 differentially regulates TRH expression in developing hypothalamic neurons in vitro Brain Research, 1305, 20-30.

Uribe,R.M. Zacarias,M. Corkidi,G. Cisneros,M. Charli,J.L. Joseph-Bravo,P. 2009. 17beta-oestradiol indirectly inhibits trh expression in the hypothalamic paraventricular nucleus of female rats and blunts thyroid axis response to cold exposure Journal of Neuroendocrinology, 21, 439-448.

Rodriguez-Molina,V. Vargas,M.A. Joseph-Bravo,P. Charli,J.L. 2009. NMDA receptor up-regulates pyroglutamyl peptidase II activity in the rat hippocampus Neuroscience Letters, 449, 211-214.

Cruz,R. Vargas,M.A. Uribe,R.M. Pascual,I. Lazcano,I. Yiotakis,A. Matziari,M. Joseph-Bravo,P. Charli,J.L. 2008. Anterior pituitary pyroglutamyl peptidase II activity controls TRH-induced prolactin release Peptides, 29, 1953-1964.

Cote-Velez,A. Perez-Martinez,L. Charli,J.L. Joseph-Bravo,P. 2008. The PKC and ERK/MAPK Pathways Regulate Glucocorticoid Action on TRH Transcription Neurochemical Research, 33, 1582-1591.

de Gortari,P. Vargas,M.A. Martinez,A. Garcia-Vazquez,A.I. Uribe,R.M. Chavez-Gutierrez,L. Magdaleno,V. Boileau,G. Charli,J.L. Joseph-Bravo,P. 2007. Stage-specific Modulation of Neprilysin and Aminopeptidase N in the Limbic System During Kindling Progression Journal of Molecular Neuroscience, 33, 252-261.

Ubieta,R. Uribe,R.M. Gonzalez,J.A. Garcia-Vazquez,A. Perez-Monter,C. Perez-Martinez,L. Joseph-Bravo,P. Charli,J.L. 2007. BDNF up-regulates pre-pro-TRH mRNA expression in the fetal/neonatal paraventricular nucleus of the hypothalamus. Properties of the transduction pathway Brain Research, 1174, 28-38.

Pascual,I. Lopez,A. Gomez,H. Chappe,M. Saroyan,A. Gonzalez,Y. Cisneros,M. Charli,J.L. Chavez,M.D.A. 2007. Screening of inhibitors of porcine dipeptidyl peptidase IV activity in aqueous extracts from marine organisms Enzyme and Microbial Technology, 40, 414-419.

Chavez-Gutierrez,L. Matta-Camacho,E. Osuna,J. Horjales,E. Joseph-Bravo,P. Maigret,B. Charli,J.L. 2006. Homology modeling and site directed mutagenesis of pyroglutamyl peptidase II. Insights into omega versus aminopeptidase specificity in the M1 family Journal of Biological Chemistry, 281, 18581-18590.

de Gortari,P. Uribe,R.M. Garcia-Vazquez,A. Aguilar-Valles,A. Martinez,A. Valdes,A. Charli,J.L. Fernandez-Guardiola,A. Joseph-Bravo,P. 2006. Amygdala kindling differentially regulates the expression of the elements involved in TRH transmission Neurochemistry International, 48, 31-42.

Cote-Velez,A. Perez-Martinez,L. Diaz-Gallardo,M.Y. Perez-Monter,C. Carreon,A. Charli,J.L. Joseph-Bravo,P. 2005. Dexamethasone repressed cAMP rapid upregulation of TRH gene transcription. Identification of a composite GRE and a CRE in TRH promoter Journal of Molecular Endocrinology, 34, 177-197.

Chavez-Gutierrez,L. Bourdais,J. Aranda,G. Vargas,M.A. Matta-Camacho,E. Ducancel,F. Segovia,L. Joseph-Bravo,P. Charli,J.L. 2005. A truncated isoform of pyroglutamyl aminopeptidase II produced by exon extension has dominant-negative activity Journal of Neurochemistry, 92, 807-817.

Pascual,I. Cisneros,M. Lage,J. Diaz,J. Joseph-Bravo,P. Charli,J.L. Chavez,M.A. 2004. Isolation and purification of HcPI, a natural inhibitor of pyroglutamil aminopeptidase II (TRH-degrading ectoenzyme) from extracts of Hermodice carunculata (annelide: Polychaeta) Revista Biologia, 18, 38-49.

Cruz,R. Chavez-Gutierrez,L. Joseph-Bravo,P. Charli,J.L. 2004. 3,3',5'-Triiodo-L-Thyronine Reduces Efficiency of mRNA Knockdown by Antisense Oligodeoxynucleotides: A Study with Pyroglutamyl Aminopeptidase II in Adenohypophysis Oligonucleotides, 14, 176-190.

Pascual,I. Gil-Parrado,S. Cisneros,M. Joseph-Bravo,P. Diaz,J. Possani,L.D. Charli,J.L. Chavez,M. 2004. Purification of a specific inhibitor of pyroglutamyl aminopeptidase II from the marine annelide Hermodice carunculata. In vivo effects in rodent brain International Journal of Biochemistry and Cell Biology, 36, 138-152.

Vargas,M.A. St Louis,M. Desgroseillers,L. Charli,J.L. Boileau,G. 2003. Parathyroid Hormone-Related Protein(1-34) Regulates Phex Expression in Osteoblasts Through the Protein Kinase A Pathway Endocrinology, 144, 4876-4885.

Guerra-Crespo,M. Charli,J.L. Rosales-Garcia,V. Pedraza-Alva,G. Perez-Martinez,L. 2003. Polyethylenimine improves the transfection efficiency of primary cultures of post-mitotic rat fetal hypothalamic neurons Journal of Neuroscience Methods, 127, 179-192.

Vargas,M.A. Uribe,R.M. Cisneros,M. Romero,F. Gonzalez,S. Joseph-Bravo,P. Charli,J.L. 2002. Thyrotropin-releasing hormone regulates the diurnal variation of pyroglutamyl aminopeptidase II activity in the male rat adenohypophysis European Journal of Endocrinology, 147, 363-369.

Vargas,M.A. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 2002. Thyrotropin-releasing hormone-induced down-regulation of pyroglutamyl aminopeptidase II activity involves L-type calcium channels and cam kinase activities in cultures of adenohypophyseal cells Journal of Neuroendocrinology, 14, 184-193.

Joseph-Bravo,P. Perez-Martinez,L. Lezama,L. Morales-Chapa,C. Charli,J.L. 2002. An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells Brain Research Protocols, 9, 93-104.

Sanchez,E. Uribe,R.M. Corkidi,G. Zoeller,R.T. Cisneros,M. Zacarias,M. Morales-Chapa,C. Charli,J.L. Joseph-Bravo,P. 2001. Differential Responses of Thyrotropin-Releasing Hormone (TRH) Neurons to Cold Exposure or Suckling Indicate Functional Heterogeneity of the TRH System in the Paraventricular Nucleus of the Rat Hypothalamus Neuroendocrinology, 74, 407-422.

Guerra-Crespo,M. Ubieta,R. Joseph-Bravo,P. Charli,J.L. Perez-Martinez,L. 2001. BDNF increases the early expression of TRH mRNA in fetal TrkB+ hypothalamic neurons in primary culture European Journal of Neuroscience, 14, 483-494.

Perez-Martinez,L. Charli,J. Joseph-Bravo,P. 2001. Development of pro-TRH gene expression in primary cultures of fetal hypothalamic cells Developmental Brain Research, 130, 73-81.

Baeza,M.A. Ponce,G. Joseph-Bravo,P. Charli,J.L. 2001. Rapid down regulation of pyroglutamyl peptidase II activity by arachidonic acid in primary cultures of adenohypophyseal cells Life Sciences, 68, 2051-2060.

Niquet,J. Perez-Martinez,L. Guerra,M. Grouselle,D. Joseph-Bravo,P. Charli,J. 2000. Extracellular matrix proteins increase the expression of pro-TRH and pro-protein convertase PC1 in fetal hypothalamic neurons in vitro Developmental Brain Research, 120, 49-56.

Niquet,J. Charli,J. 2000. In vitro expression of tyrosine hydroxylase by a subpopulation of rat melanotrophs is down-regulated by dopamine Brain Res.Bull, 51, 479-484.

Bourdais,J. Romero,F. Uriostegui,B. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 2000. [3-Me-His(2)]-TRH combined with dopamine withdrawal rapidly and transiently increases pyroglutamyl aminopeptidase II activity in primary cultures of adenohypophyseal cells Neuropeptides, 34, 83-88.

Vargas,M.A. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 2000. Regulation of adenohypophyseal pyroglutamyl aminopeptidase II activity by thyrotropin-releasing hormone and phorbol esters Endocrine, 13, 267-272.

Perez-Martinez,L. Uribe,R.M. Sanchez,E. Carreon-Rodriguez,A. Chavez,L. Zacarias,M. Joseph-Bravo,P. Charli,J.L. 1999. Regulation of TRH biosynthesis in the paraventricular nucleus of rodent hypothalamus Current topics in neurochemistry, 2, 79-88.

Mendez,M. Cisneros,M. Baez,A. Joseph-Bravo,P. Charli,J.L. 1999. Three TRH-like molecules are released from rat hypothalamus in vitro Neurochemical Research, 24, 815-823.

Niquet,J. Loudes,C. Ubieta,R. Kordon,C. Faivre-Bauman,A. Charli,J. 1999. Membranes from pituitary intermediate lobe cells enhance differentiation of fetal hypothalamic dopaminergic neurons in primary culture Developmental Brain Research, 118, 39-49.

Joseph-Bravo,P. Uribe,R.M. Vargas,M.A. Perez-Martinez,L. Zoeller,T. Charli,J.L. 1998. Multifactorial modulation of TRH metabolism Cellular & Molecular Neurobiology, 18, 231-247.

Vargas,M.A. Bourdais,J. Sanchez,S. Uriostegui,B. Moreno,E. Joseph-Bravo,P. Charli,J.L. 1998. Multiple hypothalamic factors regulate pyroglutamyl peptidase II in cultures of adenohypophyseal cells: role of the cAMP pathway Journal of Neuroendocrinology, 10, 199-206.

Charli,J.L. Vargas,M.A. Cisneros,M. de Gortari,P. Baeza,M.A. Jasso,P. Bourdais,J. Perez,L. Uribe,R.M. Joseph-Bravo,P. 1998. TRH inactivation in the extracellular compartment: role of pyroglutamyl peptidase II.Neurobiology (Bp.), 6, 45-57.

Perez-Martinez,L. Carreon-Rodriguez,A. Gonzalez-Alzati,M.E. Morales,C. Charli,J.L. Joseph-Bravo,P. 1998. Dexamethasone rapidly regulates TRH mRNA levels in hypothalamic cell cultures: interaction with the cAMP pathway Neuroendocrinology, 68, 345-354.

Sanchez,E. Charli,J.L. Morales,C. Corkidi,G. Seidah,N.G. Joseph-Bravo,P. Uribe,R.M. 1997. Expression of the proprotein convertases PC1 and PC2 mRNAs in thyrotropin releasing hormone neurons of the rat paraventricular nucleus of hypothalamus Brain Research, 761, 77-86.

Uribe,R.M. Perez-Martinez,L. Covarrubias,M.D. Gomez,O. Covarrubias,L. Charli,J.L. Joseph-Bravo,P. 1995. Phorbol ester or cAMP enhance thyrotropin-releasing hormone mRNA in primary cultures of hypothalamic cells Neuroscience Letters, 201, 41-44.

Uribe,R.M. Joseph-Bravo,P. Charli,J.L. 1995. Pups removal enhances thyrotropin-releasing hormone mRNA in the hypothalamic paraventricular nucleus European Journal of Endocrinology, 133, 354-360.

Charli,J.L. Cruz,C. Redondo,J.L. Guerra,C. Joseph-Bravo,P. 1995. Homologous conditioned medium enhances expression of TRH in hypothalamic neurons in primary culture Developmental Brain Research, 89, 155-160.

Covarrubias,L. Redondo,J.L. Vargas,M.A. Uribe,R.M. Mendez,M. Joseph-Bravo,P. Charli,J.L. 1994. In vitro TRH release from hypothalamus slices varies during the diurnal cycle Neurochemical Research, 19, 845-850.

Uribe,R.M. Joseph-Bravo,P. Ponce,G. Cisneros,M. Aceves,C. Charli,J.L. 1994. Influence of thyroid status on TRH metabolism in rat olfactory bulb Peptides, 15, 435-439.

Vargas,M.A. Joseph-Bravo,P. Charli,J.L. 1994. Thyrotropin-releasing hormone downregulates pyroglutamyl peptidase II activity in adenohypophyseal cells Neuroendocrinology, 60, 323-330.

Joseph-Bravo,P. Fresan,M. Cisneros,M. Vargas,M.A. Charli,J.L. 1994. Pyroglutamyl peptidase II activity is not in the processes of bulbospinal TRHergic neurons Neuroscience Letters, 178, 243-246.

Charli,J.L. Faivre-Bauman,A. Loudes,C. Kordon,C. 1993. Coculture of rat melanotrophs with hypothalamic cells enhances differentiation of dopaminergic neurons Mol Cell Neurosci., 4, 55-63 * .

Mendez,M. Moran,J. Wilk,S. Joseph-Bravo,P. Charli,J.L. 1993. Assessment of the role of TRH in the release of [3H]-dopamine from rat nucleus accumbens-lateral septum slices Brain Res.Bull, 31, 621-625.

Faivre-Bauman,A. Charli,J.L. Loudes,C. Kordon,C. 1993. Coculture of rat melanotrophs with fetal hypothalamic cells enhances differentiation of dopaminergic neurons Annals of the New York Academy of Sciences, 680, 505-507.

Uribe,R.M. Redondo,J.L. Charli,J.L. Joseph-Bravo,P. 1993. Suckling and cold stress rapidly and transiently increase TRH mRNA in the paraventricular nucleus Neuroendocrinology, 58, 140-145.

Vargas,M.A. Cisneros,M. Herrera,J. Joseph-Bravo,P. Charli,J.L. 1992. Regional distribution of pyroglutamyl peptidase II in rabbit brain, spinal cord, and organs Peptides, 13, 255-260.

Vargas,M.A. Herrera,J. Uribe,R.M. Charli,J.L. Joseph-Bravo,P. 1992. Ontogenesis of pyroglutamyl peptidase II activity in rat brain, adenohypophysis and pancreas Developmental Brain Research, 66, 251-256.

Uribe,R.M. Joseph-Bravo,P. Pasten,J. Ponce,G. Mendez,M. Covarrubias,L. Charli,J.L. 1991. Some events of thyrotropin-releasing hormone metabolism are regulated in lactating and cycling rats Neuroendocrinology, 54, 493-498.

Cruz,C. Charli,J.L. Vargas,M.A. Joseph-Bravo,P. 1991. Neuronal localization of pyroglutamate aminopeptidase II in primary cultures of fetal mouse brain Journal of Neurochemistry, 56, 1594-1601.

Fuchs,L.Y. Ovando,C. Joseph,P. Soberon,X. Charli,J.L. 1990. Repetitive DNA sequence from the crayfish Procambarus clarkii Nucleic Acids Research, 18, 1650.

Mengod,G. Charli,J.L. Palacios,J.M. 1990. The use of in situ hybridization histochemistry for the study of neuropeptide gene expression in the human brain Cellular & Molecular Neurobiology, 10, 113-126.

Mendez,M. Cruz,C. Joseph-Bravo,P. Wilk,S. Charli,J.L. 1990. Evaluation of the role of prolyl endopeptidase and pyroglutamyl peptidase I in the metabolism of LHRH and TRH in brain Neuropeptides, 17, 55-62.

Charli,J.L. Mendez,M. Vargas,M.A. Cisneros,M. Assai,M. Joseph-Bravo,P. Wilk,S. 1989. Pyroglutamyl peptidase II inhibition specifically increases recovery of TRH released from rat brain slices Neuropeptides, 14, 191-196.

Covarrubias,L. Uribe,R.M. Mendez,M. Charli,J.L. Joseph-Bravo,P. 1989. Hypothalamic TRH mRNA regulation under different physiological conditions Annals of the New York Academy of Sciences, 553, 476-478.

Charli,J.L. Cruz,C. Ponce,G. Vargas,M.A. Mendez,M. Cisneros,M. Pasten,J. Joseph-Bravo,P. 1989. Physiological relevance of pyroglutamate aminopeptidase II Annals of the New York Academy of Sciences, 553, 563-564.

Charli,J.L. Cruz,C. Vargas,M. Joseph-Bravo,P. 1988. The narrow specificity pyroglutamate aminopeptidase degrading TRH in brain is an ectoenzyme Neurochemistry International, 13, 237-242.

Covarrubias,L. Uribe,R.M. Mendez,M. Charli,J.L. Joseph-Bravo,P. 1988. Neuronal TRH synthesis: developmental and circadian TRH mRNA levels Biochemical and Biophysical Research Communications, 151, 615-622.

Ponce,G. Charli,J.L. Pasten,J.A. Aceves,C. Joseph-Bravo,P. 1988. Tissue-specific regulation of pyroglutamate aminopeptidase II activity by thyroid hormones Neuroendocrinology, 48, 211-213.

Mendez,M. Joseph-Bravo,P. Cisneros,M. Vargas,M.A. Charli,J.L. 1987. Regional distribution of in vitro release of thyrotropin releasing hormone in rat brain Peptides, 8, 291-298.

Charli,J.L. Mendez,M. Joseph-Bravo,P. Wilk,S. 1987. Specific inhibitors of pyroglutamyl peptidase I and prolyl endopeptidase do not change the in vitro release of TRH or its content in rodent brain Neuropeptides, 9, 373-378.

Vargas,M. Mendez,M. Cisneros,M. Joseph-Bravo,P. Charli,J.L. 1987. Regional distribution of the membrane-bound pyroglutamate amino peptidase-degrading thyrotropin-releasing hormone in rat brain Neuroscience Letters, 79, 311-314.

Saavedra,R. Bravo,P.J. Charli,J.L. Herion,P. 1987. Characterization of high affinity monoclonal antibodies against the luteinizing hormone-releasing hormone Hybridoma, 6, 663-672.

Torres,H. Charli,J.L. Vargas,M.A. Gonzalez-Noriega,A. Joseph-Bravo,P. 1986. Subcellular distribution of the enzymes degrading Thyrotropin releasing hormone and metabolites in rat brain Neurochemistry International, 9, 103-110.

Cohen,S. Charli,J.L. Diaz de Leon,L. Millar,R.P. Arimura,A. Morrison,M.R. Joseph-Bravo,P. 1986. Attempts to immunoprecipitate the LHRH precursor synthesized in cell free systems Brain Research Bulletin, 16, 309-314.

Garat,B. Miranda,J. Charli,J.L. Joseph-Bravo,P. 1985. Presence of a membrane bound pyroglutamyl amino peptidase degrading thyrotropin releasing hormone in rat brain Neuropeptides, 6, 27-40.

Charli,J.L. Ponce,G. McKelvy,J.F. Joseph-Bravo,P. 1984. Accumulation of thyrotropin releasing hormone by rat hypothalamic slices Journal of Neurochemistry, 42, 981-986.

Charli,J.L. Joseph-Bravo,P. Robert,M. 1980. Use of synthetic oligonucleotides for identification of messenger RNA for somatostatin.Boletin de estudios medicos y biologicos, 31, 220-221 * .

Joseph-Bravo,P. Charli,J.L. Sherman,T. Boyer,H. Bolivar,F. McKelvy,J.F. 1980. Identification of a putative hypothalamic mRNA coding for somatostatin and of its product in cell-free translation Biochemical and Biophysical Research Communications, 94, 1004-1012 * .

Blumberg,S. Teichberg,V.I. Charli,J.L. Hersh,L.B. McKelvy,J.F. 1980. Cleavage of substance P to an N-terminal tetrapeptide and a C-terminal heptapeptide by a post-proline Cleaving enzyme from bovine brain Brain Research, 192, 477-486 * .

Joseph-Bravo,P. Charli,J.L. Palacios,J.M. Kordon,C. 1979. Effect of neurotransmitters on the in vitro release of immunoreactive thyrotropin-releasing hormone from rat mediobasal hypothalamus Endocrinology, 104, 801-806 * .

Joseph-Bravo,P. Loudes,C. Charli,J.L. Kordon,C. 1979. Subcellular distribution of brain peptidases degrading luteinizing hormone releasing hormone (LHRH) and thyrotropin releasing hormone (TRH) Brain Research, 166, 321-329 * .

Charli,J.L. Rotsztejn,W.H. Pattou,E. Kordon,C. 1978. Effect of neurotransmitters on in vitro release of luteinizing-hormone-releasing hormone from the mediobasal hypothalamus of male rats Neurosci.Lett, 10, 159-163 * .

Charli,J.L. Joseph-Bravo,P. Palacios,J.M. Kordon,C. 1978. Histamine-induced release of thyrotropin releasing hormone from hypothalamic slices European Journal of Pharmacology, 52, 401-403 * .

Rotsztejn,W.H. Charli,J.L. Pattou,E. Kordon,C. 1977. Stimulation by dopamine of luteinizing hormone-releasing hormone (LHRH) release from the mediobasal hypothalamus in male rats Endocrinology, 101, 1475-1483 * .

Rotsztejn,W.H. Charli,J.L. Pattou,E. Epelbaum,J. Kordon,C. 1976. In vitro release of luteinizing hormone-releasing hormone (LHRH) from rat mediobasal hypothalamus: effects of potassium, calcium and dopamine Endocrinology, 99, 1663-1666 * .

Libros y capítulos recientes

Joseph-Bravo,P. Gutierrez-Mariscal,M. Jaimes-Hoy,L. Charli,J.L. 2019. Thyroid Axis and Energy Balance: Focus on Animals and Implications for Humankind en: Patel,V.B. Handbook of Famine, Starvation, and Nutrient Deprivation: From Biology to Policy. Cham, Suiza. Springer International Publishing. pags. 1099-1126

Sanchez,E. Charli,J.L. Lechan,R.M. 2013. Pyroglutamyl-peptidase II en: Rawlings,N.D. Handbook of Proteolytic Enzymes. Academic Press. pags. 414-419

Charli,J.L. Joseph-Bravo,P. 2008. El cerebro, la comunicación intercelular y los péptidos en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 5. Mexico, D.F.. UNAM. pags. 53-64

Joseph-Bravo,P. Mariscal,M. Garcia,I. Jaimes-Hoy,L. Charli,J.L. de Gortari,P. 2007. Response of limbic TRH neurons depends on the stress paradigm en: Endocrine Regulations. Int. Congress of Stress. 96-

Charli,J.L. Pascual,I. Cruz,R. Vargas,M.A. 2006. Post-Secretory Inactivation of Peptides in the Hypotalamic-Adenohypophyseal Axis. Focus on Pyroglutamyl Peptidase Ii, the Thyrotropin Releasing Hormone Inactivating Enzyme en: *joseph Molecular Endocrinology. Kerala, India,. Research Signpost. pags. 39-64

Perez-Martinez,L. Charli,J.L. 2006. Factors Involved in the Establishment of Hypothalamic Neuroendocrine Phenotypes en: *joseph Molecular Endocrinology. Kerala, India,. Research Signpost. pags. 127-147

Charli,J.L. Garat,B. Martinez-Escalera,G. Ponce,G. Miranda,J. Joseph-Bravo,P. 1984. METABOLISM OF THYROTROPIN RELEASING HORMONE AND ITS POSSIBLE RELEVANCE TO PROLACTIN SECRETION en: Valverde,R. Prolactin Secretion: a Multidisciplinary Approach. Academic Press. pags. 239-248

McKelvy,J.F. Lin,C. Chan,L. Joseph-Bravo,P. Charli,J.L. Pacheco,M. 1979. Biosynthesis of brain peptides en: Boyd,A.E. Amsterdam ; New York. Elsevier/North-Holland Biomedical Press. pags. 183-196 *

Otras Publicaciones

Guerra-Crespo,M. Charli,J.L. Perez-Martinez,L. 2005. Los arreglos de DNA: explorando la expresion genetica del cerebro Ciencia, Revista de la Academia Mexicana de Ciencia, 56, 67-76.

Charli,J.L. Ponce,G. Torres,H. Garat,B. Barquin,N. Bravo,P.J. 1983. [Cellular biochemistry of hypophysiotropic peptides. II. Liberation, action and inactivation].Boletin de estudios medicos y biologicos, 32, 243-252.

Joseph-Bravo,P. Theleen,M. de Gortari,P. Shapiro,E. Redondo,J.L. Briones,M. Merchant,H. Charli,J.L. 1983. [Biochemistry of hypophysiotropic peptides. I. Biosynthesis and its regulation].Boletin de estudios medicos y biologicos, 32, 233-241.

* Indica publicación con otra institución de adscripción