IBT-UNAM : Dr. Francisco Gonzalo Bolivar Zapata
Dr. Francisco Gonzalo Bolivar Zapata Grupo
Departamento de Ingeniería Celular y Biocatálisis
Líneas de investigación
email: bolivar @ibt.unam.mx
Teléfonos
Oficina +52 (777) 329-1860 red UNAM 27860 Laboratorio +52 (777) 329-1601 red UNAM 27601
Francisco Gonzalo Bolívar Zapata
nació en la ciudad de México en 1948. Doctor en Química por la UNAM, institución en la que es profesor e Investigador Emérito. Director fundador en 1982 del Centro de Investigación sobre Ingeniería Genética y Biotecnología de la UNAM. En 1991, la UNAM transformó a este Centro en el Instituto de Biotecnología y Bolívar fue nombrado su primer Director, cargo que ocupó hasta 1997. Ese año fue designado Coordinador de la Investigación Científica de la UNAM, puesto que ocupó por tres años. En el período 1996-2000 fungió como Vicepresidente y Presidente de la Academia Mexicana de Ciencias (AMC). Asimismo, fue miembro de la Junta Directiva de la UAM de 1997 a 2005 así como integrante de la Junta Gobierno de la UNAM de 2002 a marzo de 2012. También perteneció a la Junta de Gobierno de la Universidad Autónoma del Estado de Morelos de 2009 a 2012.
Su trabajo de investigación y desarrollo tecnológico es pionero a nivel internacional en el área de la biología molecular y la biotecnología, en particular en el aislamiento, caracterización y manipulación de genes en microorganismos. Bolívar Zapata fue miembro de un grupo de investigadores que en San Francisco, EUA, lograron por primera vez en 1977 a nivel mundial, la producción en bacterias por técnicas de ingeniería genética de proteínas transgénicas idénticas a las humanas, como la insulina que se utiliza clínicamente para contender con la diabetes. Además, su trabajo en el área de la ingeniería de vías metabólicas en microorganismos, es también pionero en el propósito de la modificación genética y de la fisiología bacteriana, para el diseño y la optimización de microorganismos productores de metabolitos y proteínas de interés social y comercial.
El Dr. Bolívar cuenta con más de 240 publicaciones citadas más de 14,500 veces en la literatura mundial, incluyendo aquí 850 citas en 330 libros de texto y especializados. Ha dirigido más de 65 tesis, siendo la mayoría de posgrado; muchos de sus alumnos son profesores-investigadores y técnicos en instituciones nacionales e internacionales. Ha escrito y editado libros de divulgación y opinión, incluyendo cinco tomos de su obra científica como miembro de El Colegio Nacional.
- Licenciatura: Química, Fac. de Química-UNAM (1971)
Doctorado Honoris Causa Colegio de Postgraduados (2014) Coordinador de Ciencia, Tecnología e Innovación Oficina de la Presidencia de la República (2013 a la fecha) Doctorado Honoris Causa Universidad Autónoma Metropolitana (2008) Miembro de la Junta de Gobierno del CONACyT (2007 a la fecha) Miembro de la Junta de Gobierno de la UAEM (2006-2012) Investigador emérito UNAM (2005) Miembro de la Junta de Gobierno de la UNAM (2002-2012) Presidente de la Academia Mexicana de Ciencias (1998-2000) Premio Luis Elizondo Instituto Tecnológico de Estudios Superiores de Monterrey (1998) Premio TWAS The Third Word Academy of Sciences (1997) Coordinador de la Investigación Científica de la UNAM (1997-2000) Miembro de la Junta Directiva de la Universidad Autónoma Metropolitana (1996-2005) Miembro vitalicio de El Colegio Nacional (1994) Doctorado Honoris Causa Universida de Lieja, Belgica (1994) Medalla Alfonso Herrera Universidad Autónoma de Puebla (1993) Premio Nacional de Ciencias y Artes Gobierno de la República (1992) Premio Cecilio A. Robelo UAEM y Gobierno del Estado de Morelos (1992) Primer Director del Instituto de Biotecnología de la UNAM (1991-1997) Premio Principe de Asturias en Investigación Científica y Técnica (1991) Premio Universidad Nacional en el área de investigación en Ciencias Naturales UNAM (1990) Premio Manuel Noriega en Ciencias Biológicas OEA (1988) Premio de la Academia de la Investigación Científica en el área de Ciencias Naturales (1982) Director Fundador del Centro de Investigación sobre Ingeniería Genética y Biotecnología de la UNAM (1982-1991) Premio Nacional de Química Gobierno Federal (1980)
Estudiantes M.C. Himmer Soto
Jose de Jesus Gonzalez
Publicaciones recientes
Moreno-Avitia,F. Utrilla,J. Bolivar,F. Nogales,J. Escalante,A.
2020. Metabolic reconstruction of Pseudomonas chlororaphis ATCC 9446 to understand its metabolic potential as a phenazine-1-carboxamide-producing strain Applied Microbiology and Biotechnology, 104, 10119-10132.
Carmona,S.B. Flores,N. Martinez-Romero,E. Gosset,G. Bolivar,F. Escalante,A.
2020. Evolution of an Escherichia coli PTS(-) strain: a study of reproducibility and dynamics of an adaptive evolutive process Applied Microbiology and Biotechnology, 104, 9309-9325.
Alva,A. Sabido-Ramos,A. Escalante,A. Bolivar,F.
2020. New insights into transport capability of sugars and its impact on growth from novel mutants of Escherichia coli Applied Microbiology and Biotechnology, 104, 1463-1479.
Fernandez-Sandoval,M.T. Galindez-Mayer,J. Bolivar,F. Gosset,G. Ramirez,O.T. Martinez,A.
2019. Xylose-glucose co-fermentation to ethanol by Escherichia coli strain MS04 using single- and two-stage continuous cultures under micro-aerated conditions Microbial Cell Factories, 18, 145.
Martinez,J.A. Rodriguez,A. Moreno,F. Flores,N. Lara,A.R. Ramirez,O.T. Gosset,G. Bolivar,F.
2018. Metabolic modeling and response surface analysis of an Escherichia coli strain engineered for shikimic acid production BMC Systems Biology, 12, 102.
Aguilar,C. Martinez-Batallar,G. Flores,N. Moreno-Avitia,F. Encarnacion,S. Escalante,A. Bolivar,F.
2018. Analysis of differentially upregulated proteins in ptsHIcrr(-) and rppH(-) mutants in Escherichia coli during an adaptive laboratory evolution experiment Applied Microbiology and Biotechnology, 102, 10193-10208.
Salas-Navarrete,C. Hernandez-Chavez,G. Flores,N. Martinez,L.M. Martinez,A. Bolivar,F. Barona-Gomez,F. Gosset,G.
2018. Increasing pinosylvin production in Escherichia coli by reducing the expression level of the gene fabI-encoded enoyl-acyl carrier protein reductase Electronic Journal of Biotechnology, 33, 11-16.
Diaz-Quiroz,D.C. Cardona-Felix,C.S. Viveros-Ceballos,J.L. Reyes-Gonzalez,M.A. Bolivar,F. Ordonez,M. Escalante,A.
2018. Synthesis, biological activity and molecular modelling studies of shikimic acid derivatives as inhibitors of the shikimate dehydrogenase enzyme of Escherichia coli Journal of Enzyme Inhibition and Medicinal Chemistry, 33, 397-404.
Moreno-Avitia,F. Lozano,L. Utrilla,J. Bolivar,F. Escalante,A.
2017. Draft Genome Sequence of Pseudomonas chlororaphis ATCC 9446, a Nonpathogenic Bacterium with Bioremediation and Industrial Potential Genome Announcements, 5, .
Rodriguez,A. Martinez,J.A. Millard,P. Gosset,G. Portais,J.C. Letisse,F. Bolivar,F.
2017. Plasmid-encoded biosynthetic genes alleviate metabolic disadvantages while increasing glucose conversion to shikimate in an engineered Escherichia coli strain Biotechnology and Bioengineering, 114, 1319-1330.
Huerta-Beristain,G. Cabrera-Ruiz,R. Hernandez-Chavez,G. Bolivar,F. Gosset,G. Martinez,A.
2017. Metabolic engineering and adaptive evolution of Escherichia coli KO11 for ethanol production through the Entner-Doudoroff and the pentose phosphate pathways Journal of Chemical Technology and Biotechnology, 92, 990-996.
Garcia,S. Flores,N. de Anda,R. Hernandez,G. Gosset,G. Bolivar,F. Escalante,A.
2017. The Role of the ydiB Gene, Which Encodes Quinate/Shikimate Dehydrogenase, in the Production of Quinic, Dehydroshikimic and Shikimic Acids in a PTS- Strain of Escherichia coli Journal of Molecular Microbiology and Biotechnology, 27, 11-21.
Camacho-Zaragoza,J.M. Hernandez-Chavez,G. Moreno-Avitia,F. Ramirez-Iniguez,R. Martinez,A. Bolivar,F. Gosset,G.
2016. Engineering of a microbial coculture of Escherichia coli strains for the biosynthesis of resveratrol Microbial Cell Factories, 15, 163.
Mejia-Caballero,A. de Anda,R. Hernandez-Chavez,G. Rogg,S. Martinez,A. Bolivar,F. Castano,V.M. Gosset,G.
2016. Biosynthesis of catechol melanin from glycerol employing metabolically engineered Escherichia coli Microbial Cell Factories, 15, 161.
Giles-Gomez,M. Sandoval-Garcia,J.G. Matus,V. Campos-Quintana,I. Bolivar,F. Escalante,A.
2016. In vitro and in vivo probiotic assessment of Leuconostoc mesenteroides P45 isolated from pulque, a Mexican traditional alcoholic beverage Springerplus, 5, 708.
Escalante,A. Lopez-Soto,D.R. Velazquez-Gutierrez,J.E. Giles-Gomez,M. Bolivar,F. Lopez-Munguia,A.
2016. Pulque, a Traditional Mexican Alcoholic Fermented Beverage: Historical, Microbiological, and Technical Aspects Frontiers in Microbiology, 7, 1026.
Cortes,J.T. Flores,N. Bolivar,F. Lara,A.R. Ramirez,O.T.
2016. Physiological effects of pH gradients on Escherichia coli during plasmid DNA production Biotechnology and Bioengineering, 113, 598-611.
Martinez,J.A. Bolivar,F. Escalante,A.
2015. Shikimic Acid Production in Escherichia coli: From Classical Metabolic Engineering Strategies to Omics Applied to Improve Its Production Frontiers in Bioengineering and Biotechnology, 3, 145.
Aguilar,C. Flores,N. Riveros-McKay,F. Sahonero-Canavesi,D. Carmona,S.B. Geiger,O. Escalante,A. Bolivar,F.
2015. Deletion of the 2-acyl-glycerophosphoethanolamine cycle improve glucose metabolism in Escherichia coli strains employed for overproduction of aromatic compounds Microbial Cell Factories, 14, 194.
Carmona,S.B. Moreno,F. Bolivar,F. Gosset,G. Escalante,A.
2015. Inactivation of the PTS as a Strategy to Engineer the Production of Aromatic Metabolites in Escherichia coli Journal of Molecular Microbiology and Biotechnology, 25, 195-208.
Vargas-Tah,A. Martinez,L. Hernandez-Chavez,G. Rocha,M. Martinez,A. Bolivar,F. Gosset,G.
2015. Production of cinnamic and p -hydroxycinnamic acid from sugar mixtures with engineered Escherichia coli Microbial Cell Factories, 14, 6.
Riveros-McKay,F. Campos,I. Giles-Gomez,M. Bolivar,F. Escalante,A.
2014. Draft Genome Sequence of Leuconostoc mesenteroides P45 Isolated from Pulque, a Traditional Mexican Alcoholic Fermented Beverage Genome Announcements, 2, .
Torres-Rodriguez,I. Rodriguez-Alegria,M.E. Miranda-Molina,A. Giles-Gomez,M. Conca-Morales R. Lopez-Munguia,A. Bolivar,F. Escalante,A.
2014. Screening and characterization of extracellular polysaccharides produced by Leuconostoc kimchii isolated from traditional fermented pulque beverage Springerplus, 3, 583.
Balderas-Hernandez,V.E. Trevino-Quintanilla,L.G. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G.
2014. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1 Microbial Cell Factories, 13, 136.
Diaz-Quiroz,D.C. Carmona,S.B. Bolivar,F. Escalante,A.
2014. Current perspectives on applications of shikimic and aminoshikimic acids in pharmaceutical chemistry Research and Reports in Medicinal Chemistry, 4, 35-46.
Rodriguez,A. Martinez,J.A. Flores,N. Escalante,A. Gosset,G. Bolivar,F.
2014. Engineering Escherichia coli to overproduce aromatic amino acids and derived compounds Microbial Cell Factories, 13, 126.
Cortes-Tolalpa,L. Gutierrez-Rios,R.M. Martinez,L.M. de Anda,R. Gosset,G. Bolivar,F. Escalante,A.
2014. Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium Microbial Cell Factories, 13, 28.
Centeno-Leija,S. Huerta-Beristain,G. Giles-Gomez,M. Bolivar,F. Gosset,G. Martinez,A.
2014. Improving poly-3-hydroxybutyrate production in Escherichia coli by combining the increase in the NADPH pool and acetyl-CoA availability Antonie Van Leeuwenhoek, 105, 687-696.
Sabido,A. Sigala,J.C. Hernandez-Chavez,G. Flores,N. Gosset,G. Bolivar,F.
2014. Physiological and transcriptional characterization of Escherichia coli strains lacking interconversion of phosphoenolpyruvate and pyruvate when glucose and acetate are coutilized Biotechnology and Bioengineering, 111, 1150-1160.
Licona-Cassani,C. Lara,A.R. Cabrera-Valladares,N. Escalante,A. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G.
2014. Inactivation of Pyruvate Kinase or the Phosphoenolpyruvate: Sugar Phosphotransferase System Increases Shikimic and Dehydroshikimic Acid Yields from Glucose in Bacillus subtilis Journal of Molecular Microbiology and Biotechnology, 24, 37-45.
Chavez-Bejar,M.I. Balderas-Hernandez,V.E. Gutierrez-Alejandre,A. Martinez,A. Bolivar,F. Gosset,G.
2013. Metabolic engineering of Escherichia coli to optimize melanin synthesis from glucose Microbial Cell Factories, 12, 108.
Rodriguez,A. Martinez,J.A. Baez-Viveros,J.L. Flores,N. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Bolivar,F.
2013. Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF Microbial Cell Factories, 12, 86.
Caspeta,L. Lara,A.R. Perez,N.O. Flores,N. Bolivar,F. Ramirez,O.T.
2013. Enhancing thermo-induced recombinant protein production in Escherichia coli by temperature oscillations and post-induction nutrient feeding strategies Journal of Biotechnology, 167, 47-55.
Fuentes,L.G. Lara,A.R. Martinez,L.M. Ramirez,O.T. Martinez,A. Bolivar,F. Gosset,G.
2013. Modification of glucose import capacity in Escherichia coli: physiologic consequences and utility for improving DNA vaccine production Microbial Cell Factories, 12, 42.
Soria,S. de Anda,R. Flores,N. Romero-Garcia,S. Gosset,G. Bolivar,F. Baez-Viveros,J.L.
2013. New insights on transcriptional responses of genes involved in carbon central metabolism, respiration and fermentation to low ATP levels in Escherichia coli Journal of Basic Microbiology, 53, 365-380.
Sabido,A. Martinez,L.M. de Anda,R. Martinez,A. Bolivar,F. Gosset,G.
2013. A novel plasmid vector designed for chromosomal gene integration and expression: Use for developing a genetically stable Escherichia coli melanin production strain Plasmid, 69, 16-23.
Meza,E. Becker,J. Bolivar,F. Gosset,G. Wittmann,C.
2012. Consequences of phosphoenolpyruvate:sugar phosphotranferase system and pyruvate kinase isozymes inactivation in central carbon metabolism flux distribution in Escherichia coli Microbial Cell Factories, 11, 127.
Aguilar,C. Escalante,A. Flores,N. de Anda,R. Riveros-McKay,F. Gosset,G. Morett,E. Bolivar,F.
2012. Genetic changes during a laboratory adaptive evolution process that allowed fast growth in glucose to an Escherichia coli strain lacking the major glucose transport system BMC Genomics, 13, 385.
Cabrera-Valladares,N. Martinez,L.M. Flores,N. Hernandez-Chavez,G. Martinez,A. Bolivar,F. Gosset,G.
2012. Physiologic Consequences of Glucose Transport and Phosphoenolpyruvate Node Modifications in Bacillus subtilis 168 Journal of Molecular Microbiology and Biotechnology, 22, 177-197.
Fernandez-Sandoval,M.T. Huerta-Beristain,G. Trujillo-Martinez,B. Bustos,P. Gonzalez,V. Bolivar,F. Gosset,G. Martinez,A.
2012. Laboratory metabolic evolution improves acetate tolerance and growth on acetate of ethanologenic Escherichia coli under non-aerated conditions in glucose-mineral medium Applied Microbiology and Biotechnology, 96, 1291-1300.
Chavez-Bejar,M.I. Baez-Viveros,J.L. Martinez,A. Bolivar,F. Gosset,G.
2012. Biotechnological production of l-tyrosine and derived compounds Process Biochemistry, 47, 1017-1026.
Escalante,A. Salinas-Cervantes A. Gosset,G. Bolivar,F.
2012. Current knowledge of the Escherichia coli phosphoenolpyruvate-carbohydrate phosphotransferase system: peculiarities of regulation and impact on growth and product formation Applied Microbiology and Biotechnology, 94, 1483-1494.
Martinez-Gomez,K. Flores,N. Castaneda,H.M. Martinez-Batallar,G. Hernandez-Chavez,G. Ramirez,O.T. Gosset,G. Encarnacion,S. Bolivar,F.
2012. New insights into Escherichia coli metabolism: carbon scavenging, acetate metabolism and carbon recycling responses during growth on glycerol Microbial Cell Factories, 11, 46.
Baez,A. Flores,N. Bolivar,F. Ramirez,O.T.
2011. Simulation of dissolved CO(2) gradients in a scale-down system: A metabolic and transcriptional study of recombinant Escherichia coli Biotechnology Journal, 6, 959-967.
Munoz,A.J. Hernandez-Chavez,G. de Anda,R. Martinez,A. Bolivar,F. Gosset,G.
2011. Metabolic engineering of Escherichia coli for improving L: -3,4-dihydroxyphenylalanine (L: -DOPA) synthesis from glucose Journal of Industrial Microbiology & Biotechnology, 38, 1845-1852.
Balderas-Hernandez,V.E. Hernandez-Montalvo,V. Bolivar,F. Gosset,G. Martinez,A.
2011. Adaptive Evolution of Escherichia coli Inactivated in the Phosphotransferase System Operon Improves Co-utilization of Xylose and Glucose Under Anaerobic Conditions Applied Biochemistry and Biotechnology, 163, 485-496.
Escalante,A. Calderon,R. Valdivia,A. de Anda,R. Hernandez,G. Ramirez,O.T. Gosset,G. Bolivar,F.
2010. Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Microbial Cell Factories, 9, 21.
Olvera,L. Mendoza-Vargas,A. Flores,N. Olvera,M. Sigala,J.C. Gosset,G. Morett,E. Bolivar,F.
2009. Transcription Analysis of Central Metabolism Genes in Escherichia coli. Possible Roles of a38 in Their Expression, as a Response to Carbon Limitation PLoS ONE, 4, e7466.
Baez,A. Flores,N. Bolivar,F. Ramirez,O.T.
2009. Metabolic and transcriptional response of recombinant Escherichia coli to elevated dissolved carbon dioxide concentrations Biotechnology and Bioengineering, 104, 102-110.
Balderas-Hernandez,V.E. Sabido-Ramos,A. Silva,P. Cabrera-Valladares,N. Hernandez-Chavez,G. Baez-Viveros,J.L. Martinez,A. Bolivar,F. Gosset,G.
2009. Metabolic engineering for improving anthranilate synthesis from glucose in Escherichia coli Microbial Cell Factories, 8, 19.
Caspeta,L. Flores,N. Perez,N.O. Bolivar,F. Ramirez,O.T.
2009. The effect of heating rate on Escherichia coli metabolism, physiological stress, transcriptional response, and production of temperature-induced recombinant protein: a scale-down study Biotechnology and Bioengineering, 102, 468-482.
Sigala,J.C. Flores,S. Flores,N. Aguilar,C. de Anda,R. Gosset,G. Bolivar,F.
2009. Acetate Metabolism in Escherichia coli Strains Lacking Phosphoenolpyruvate: Carbohydrate Phosphotransferase System; Evidence of Carbon Recycling Strategies and Futile Cycles Journal of Molecular Microbiology and Biotechnology, 16, 224-235.
Orencio-Trejo,M. Flores,N. Escalante,A. Hernandez-Chavez,G. Bolivar,F. Gosset,G. Martinez,A.
2008. Metabolic regulation analysis of an ethanologenic Escherichia coli strain based on RT-PCR and enzymatic activities Biotechnology for Biofuels, 1, 8.
Escalante,A. Giles-Gomez,M. Hernandez,G. Cordova-Aguilar,M.S. Lopez-Munguia,A. Gosset,G. Bolivar,F.
2008. Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach International Journal of Food Microbiology, 124, 126-134.
Huerta-Beristain,G. Utrilla,J. Hernandez-Chavez,G. Bolivar,F. Gosset,G. Martinez,A.
2008. Specific ethanol production rate in ethanologenic Escherichia coli strain KO11 Is limited by pyruvate decarboxylase Journal of Molecular Microbiology and Biotechnology, 15, 55-64.
Chavez-Bejar,M.I. Lara,A.R. Lopez,H. Hernandez-Chavez,G. Martinez,A. Ramirez,O.T. Bolivar,F. Gosset,G.
2008. Metabolic engineering of Escherichia coli for L-tyrosine production by the expression of the genes coding for the chorismate mutase domain from native P-protein and a cyclohexadienyl dehydrogenase from Zymomonas mobilis Applied and Environmental Microbiology, 74, 3284-3290.
Martinez,K. de Anda,R. Hernandez,G. Escalante,A. Gosset,G. Ramirez,O.T. Bolivar,F.G.
2008. Coutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system Microbial Cell Factories, 7, 1.
Flores,N. Escalante,A. de Anda,R. Baez-Viveros,J.L. Merino,E. Franco,B. Georgellis,D. Gosset,G. Bolivar,F.
2008. New Insights into the Role of Sigma Factor RpoS as Revealed in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Journal of Molecular Microbiology and Biotechnology, 14, 176-192.
Lara,A.R. Caspeta,L. Gosset,G. Bolivar,F. Ramirez,O.T.
2008. Utility of an Escherichia coli strain engineered in the substrate uptake system for improved culture performance at high glucose and cell concentrations: An alternative to fed-batch cultures Biotechnology and Bioengineering, 99, 893-901.
Baez-Viveros,J.L. Flores,N. Suarez,K. Castillo-Espana,P. Bolivar,F. Gosset,G.
2007. Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine Microbial Cell Factories, 6, 30.
Pinero,S. Rivera,J. Romero,D. Cevallos,M.A. Martinez,A. Bolivar,F. Gosset,G.
2007. Tyrosinase from Rhizobium etli Is Involved in Nodulation Efficiency and Symbiosis-Associated Stress Resistance Journal of Molecular Microbiology and Biotechnology, 13, 35-44.
Flores,N. Leal,L. Sigala,J.C. de Anda,R. Escalante,A. Martinez,A. Ramirez,O.T. Gosset,G. Bolivar,F.
2007. Growth Recovery on Glucose under Aerobic Conditions of an Escherichia coli Strain Carrying a Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Deletion by Inactivating arcA and Overexpressing the Genes Coding for Glucokinase and Galactose Permease Journal of Molecular Microbiology and Biotechnology, 13, 105-116.
Trevino-Quintanilla,L.G. Escalante,A. Caro,A.D. Martinez,A. (error para richar) Puente,J.L. Bolivar,F. Gosset,G.
2007. The Phosphotransferase System-Dependent Sucrose Utilization Regulon in Enteropathogenic Escherichia coli Strains Is Located in a Variable Chromosomal Region Containing iap Sequences Journal of Molecular Microbiology and Biotechnology, 13, 117-125.
Romero,S. Merino,E. Bolivar,F. Gosset,G. Martinez,A.
2007. Metabolic engineering of Bacillus subtilis for ethanol production: Lactate dehydrogenase plays a key role in the fermentative metabolism Applied and Environmental Microbiology, 73, 5190-5198.
Lagunas-Munoz,V.H. Cabrera-Valladares,N. Bolivar,F. Gosset,G. Martinez,A.
2006. Optimum melanin production using recombinant Escherichia coli Journal of Applied Microbiology, 101, 1002-1008.
Lara,A.R. Vazquez-Limon,C. Gosset,G. Bolivar,F. Lopez-Mungua,A. Ramirez,O.T.
2006. Engineering Escherichia coli to improve culture performance and reduce formation of by-products during recombinant protein production under transient intermittent anaerobic conditions Biotechnology and Bioengineering, 94, 1164-1175.
Cabrera-Valladares,N. Martinez,A. Pinero,S. Lagunas-Munoz,V.H. Tinoco,R. de Anda,R. Vazquez-Duhalt,R. Bolivar,F. Gosset,G.
2006. Expression of the melA gene from Rhizobium etli CFN42 in Escherichia coli and characterization of the encoded tyrosinase Enzyme and Microbial Technology, 38, 772-779.
de Anda,R. Lara,A.R. Hernandez,V. Hernandez-Montalvo,V. Gosset,G. Bolivar,F. Ramirez,O.T.
2006. Replacement of the glucose phosphotransferase transport system by galactose permease reduces acetate accumulation and improves process performance of Escherichia coli for recombinant protein production without impairment of growth rate Metabolic Engineering, 8, 281-290.
Lara,A.R. Leal,L. Flores,N. Gosset,G. Bolivar,F. Ramirez,O.T.
2006. Transcriptional and metabolic response of recombinant Escherichia coli to spatial dissolved oxygen tension gradients simulated in a scale-down system Biotechnology and Bioengineering, 93, 372-385.
Flores,S. Flores,N. de Anda,R. Gonzalez,A. Escalante,A. Sigala,J.C. Gosset,G. Bolivar,F.
2005. Nutrient-Scavenging Stress Response in an Escherichia coli Strain Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System, as Explored by Gene Expression Profile Analysis Journal of Molecular Microbiology and Biotechnology, 10, 51-63.
Flores,N. Flores,S. Escalante,A. de Anda,R. Leal,L. Malpica,R. Georgellis,D. Gosset,G. Bolivar,F.
2005. Adaptation for fast growth on glucose by differential expression of central carbon metabolism and gal regulon genes in an Escherichia coli strain lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system Metabolic Engineering, 7, 70-87.
Sandoval-Basurto,E.A. Gosset,G. Bolivar,F. Ramirez,O.T.
2005. Culture of Escherichia coli under dissolved oxygen gradients simulated in a two-compartment scale-down system: Metabolic response and production of recombinant protein Biotechnology and Bioengineering, 89, 453-463.
Flores,N. de Anda,R. Flores,S. Escalante,A. Hernandez,G. Martinez,A. Ramirez,O.T. Gosset,G. Bolivar,F.
2004. Role of Pyruvate Oxidase in Escherichia coli Strains Lacking the Phosphoenolpyruvate:Carbohydrate Phosphotransferase System Journal of Molecular Microbiology and Biotechnology, 8, 209-221.
Flores,S. Anda-Herrera,R. Gosset,G. Bolivar,F.G.
2004. Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway Biotechnology and Bioengineering, 87, 485-494.
Baez-Viveros,J.L. Osuna,J. Hernandez-Chavez,G. Soberon,X. Bolivar,F. Gosset,G.
2004. Metabolic engineering and protein directed evolution increase the yield of L-phenylalanine synthesized from glucose in Escherichia coli Biotechnology and Bioengineering, 87, 516-524.
Escalante,A. Rodriguez,M.E. Martinez,A. Lopez-Munguia,A. Bolivar,F. Gosset,G.
2004. Characterization of bacterial diversity in Pulque, a traditional Mexican alcoholic fermented beverage, as determined by 16S rDNA analysis Fems Microbiology Letters, 235, 273-279.
Hernandez-Montalvo,V. Martinez,A. Bolivar,F. Valle,F. Gosset,G.
2003. Expression of galP and glk in a Escherichia coli PTS mutant restores glucose transport and increases glycolytic flux to fermentation products Biotechnology and Bioengineering, 83, 687-694.
Flores,S. Gosset,G. Flores,N. de Graaf,A.A. Bolivar,F.
2002. Analysis of carbon metabolism in Escherichia coli strains with an inactive phosphotransferase system by 13C labelling and NMR spectroscopy Metabolic Engineering, 4, 124-137.
Hernandez-Montalvo,V. Valle,F. Bolivar,F. Gosset,G.
2001. Characterization of sugar mixtures utilization by an Escherichia coli mutant devoid of the phosphotransferase system Applied Microbiology and Biotechnology, 57, 186-191.
Le Borgne,S. Palmeros,B. Bolivar,F. Gosset,G.
2001. Improvement of the pBRINT-Ts plasmid family to obtain marker-free chromosomal insertion of cloned DNA in E. coli Biotechniques, 30, 252-256.
Jan,J. Valle,F. Bolivar,F. Merino,E.
2001. Construction of protein overproducer strains in Bacillus subtilis by an integrative approach Applied Microbiology and Biotechnology, 55, 69-75.
Baez,J.L. Bolivar,F. Gosset,G.
2001. Determination of 3-deoxy-D-arabino-heptulosonate 7-phosphate productivity and yield from glucose in Escherichia coli devoid of the glucose phosphotransferase transport system Biotechnology and Bioengineering, 73, 530-535.
Possani,L.D. Merino,E. Corona,M. Bolivar,F. Becerril,B.
2000. Peptides and genes coding for scorpion toxins that affect ion-channels Biochimie, 82, 861-868.
Palmeros,B. Wild,J. Szybalski,W. Le Borgne,S. Hernandez-Chavez,G. Gosset,G. Valle,F. Bolivar,F.
2000. A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria Gene, 247, 255-264.
Jauregui,R. Bolivar,F. Merino,E.
2000. Relationship between whole proteome aminoacid composition and static DNA curvature Microbial & comparative genomics, 5, 7-15.
Jan,J. Valle,F. Bolivar,F. Merino,E.
2000. Characterization of the 5 ' subtilisin (aprE) regulatory region from Bacillus subtilis Fems Microbiology Letters, 183, 9-14.
Cruz,N. Le Borgne,S. Hernandez-Chavez,G. Gosset,G. Valle,F. Bolivar,F.
2000. Engineering the Escherichia coli outer membrane protein OmpC for metal bioadsorption Biotechnology Letters, 22, 623-629.
Siguenza,R. Flores,N. Hernandez,G. Martinez,A. Bolivar,F. Valle,F.
1999. Kinetic characterization in batch and continuous culture of Escherichia coli mutants affected in phosphoenolpyruvate metabolism: differences in acetic acid production World Journal Of Microbiology & Biotechnology, 15, 587-592.
Le Borgne,S. Palmeros,B. Valle,F. Bolivar,F. Gosset,G.
1998. pBRINT-Ts: a plasmid family with a temperature-sensitive replicon, designed for chromosomal integration into the lacZ gene of Escherichia coli Gene, 223, 213-219.
Jauregui,R. O'Reilly,F. Bolivar,F. Merino,E.
1998. Relationship between codon usage and sequence-dependent curvature of genomes Microbial & comparative genomics, 3, 243-253.
Ponce,E. Martinez,A. Bolivar,F. Valle,F.
1998. 3.0.CO;2-D> Stimulation of glucose catabolism through the pentose pathway by the absence of the two pyruvate kinase isoenzymes in Escherichia coli Biotechnology and Bioengineering, 58, 292-295.
Olmos,J. de Anda,R. Ferrari,E. Bolivar,F. Valle,F.
1997. Effects of the sinR and degU32 (Hy) mutations on the regulation of the aprE gene in Bacillus subtilis Molecular and General Genetics, 253, 562-567.
Bolivar,F.
1997. Biotechnology in Mexico: planning for the future Nature Biotechnology, 15, 742-743.
Olmos,J. Bolanos,V. Causey,S. Ferrari,E. Bolivar,F. Valle,F.
1996. A functional Spo0A is required for maximal aprE expression in Bacillus subtilis FEBS Letters, 381, 29-31.
Balbas,P. Alexeyev,M. Shokolenko,I. Bolivar,F. Valle,F.
1996. A pBRINT family of plasmids for integration of cloned DNA into the Escherichia coli chromosome Gene, 172, 65-69.
Flores,N. Xiao,J. Berry,A. Bolivar,F. Valle,F.
1996. Pathway engineering for the production of aromatic compounds in Escherichia coli Nature Biotechnology, 14, 620-623.
Valle,F. Munoz,E. Ponce,E. Flores,N. Bolivar,F.
1996. Basic and applied aspects of metabolic diversity: The phosphoenolpyruvate node Journal of Industrial Microbiology & Biotechnology, 17, 458-462.
Ponce,E. Flores,N. Martinez,A. Valle,F. Bolivar,F.
1995. Cloning of the two pyruvate kinase isoenzyme structural genes from Escherichia coli: the relative roles of these enzymes in pyruvate biosynthesis Journal of Bacteriology, 177, 5719-5722.
Becerril,B. Corona,M. Garcia,C. Bolivar,F. Possani,L.D.
1995. Cloning of genes encoding scorpion toxins: An interpretative review Journal Of Toxicology-Toxin Reviews, 14, 339-357.
Bolivar,F. Galindo,E. Lopez-Munguia,A. Quintero,R.
1994. The Institute of Biotechnology at the National-University-Of-Mexico Process Biochemistry, 29, 177-180.
Merino,E. Balbas,P. Puente,J.L. Bolivar,F.
1994. Antisense overlapping open reading frames in genes from bacteria to humans Nucleic Acids Research, 22, 1903-1908.
Olmos,J. Cruz,N. Sanchez,M. Lopez,M. Balbas,P. Gosset,G. Valle,F. Bolivar,F.
1994. Production in Escherichia coli of a rat chimeric proinsulin polypeptide carrying human A and B chains and its preparative chromatography Journal of Biotechnology, 38, 89-96.
Ramirez,O.T. Zamora,R. Espinosa,G. Merino,E. Bolivar,F. Quintero,R.
1994. Kinetic study of penicillin acylase production by recombinant E. Coli in batch cultures Process Biochemistry, 29, 197-206.
Vazquez,A. Becerril,B. Martin,B.M. Zamudio,F. Bolivar,F. Possani,L.D.
1993. Primary structure determination and cloning of the cDNA encoding toxin 4 of the scorpion Centruroides noxius Hoffmann FEBS Letters, 320, 43-46.
Becerril,B. Vazquez,A. Garcia,C. Corona,M. Bolivar,F. Possam,L.D.
1993. Cloning and characterization of cDNAs that code for Na(+)-channel-blocking toxins of the scorpion Centruroides noxius Hoffmann Gene, 128, 165-171.
Becerril,B. Corona,M. Mejia,M.C. Martin,B.M. Lucas,S. Bolivar,F. Possani,L.D.
1993. The genomic region encoding toxin gamma from the scorpion Tityus serrulatus contains an intron FEBS Letters, 335, 6-8.
Balbas,P. Alvarado,X. Bolivar,F. Valle,F.
1993. Plasmid pBRINT: a vector for chromosomal insertion of cloned DNA Gene, 136, 211-213.
Gosset,G. de Anda,R. Cruz,N. Martinez,A. Quintero,R. Bolivar,F.
1993. Recombinant protein production in cultures of an Escherichia coli trp- strain Applied Microbiology and Biotechnology, 39, 541-546.
Bolivar,F.
1993. The ethical and moral aspects of genetics research Salud Publica de Mexico, 35, 714-718.
Merino,E. Osuna,J. Bolivar,F. Soberon,X.
1992. A general, PCR-based method for single or combinatorial oligonucleotide-directed mutagenesis on pUC/M13 vectors.Biotechniques, 12, 508-510.
Castano,I. Flores,N. Valle,F. Covarrubias,A.A. Bolivar,F.
1992. gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression Molecular Microbiology, 6, 2733-2741.
Itakura,K. Tadaaki,H. Crea,R. Riggs,A.D. Heyneker,H.L. Bolivar,F. Boyer,H.W.
1992. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin. 1977.Biotechnology (Reading, Mass.), 24, 84-91.
Bolivar,F. Rodriguez,R.L. Greene,P.J. Betlach,M.C. Heynker,H.L. Boyer,H.W. Crosa,J.H. Falkow,S.
1992. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.Biotechnology (Reading, Mass.), 24, 153-171.
Flores,N. Valle,F. Bolivar,F. Merino,E.
1992. Recovery of DNA from agarose gels stained with methylene blue.Biotechniques, 13, 203-205.
Merino,E. Balbas,P. Aarons,S.R. Recillas,F. Becerril,B. Valle,F. Bolivar,F.
1992. Carbon regulation and the role in nature of the Escherichia coli penicillin acylase (pac) gene Molecular Microbiology, 6, 2175-2182.
Cruz,N. Lopez,M. Estrada,G. Alvarado,X. de Anda,R. Balbas,P. Gosset,G. Bolivar,F.
1992. Preparative isolation of recombinant human insulin-A chain by ion exchange chromatography Journal Of Liquid Chromatography, 15, 2311-2324.
Merino,E. Balbas,P. Bolivar,F.
1991. New insights on the comma-less theory Origins of Life and Evolution of the Biosphere, 21, 251-254.
Valle,F. Balbas,P. Merino,E. Bolivar,F.
1991. The role of penicillin amidases in nature and in industry Trends in Biochemical Sciences, 16, 36-40.
Cruz,N. Antonio,S. de Anda,R. Gosset,G. Bolivar,F.
1990. Preparative isolation by high performance liquid chromatography of human insulin B chain produced in Escherichia coli Journal Of Liquid Chromatography, 13, 1517-1528.
Galindo,E. Bolivar,F. Quintero,R.
1990. Maximizing the expression of recombinant proteins in Escheria coli by manipulation of culture conditions Journal Of Fermentation And Bioengineering, 69, 159-165.
Merino,E. Bolivar,F.
1989. The ribonucleoside diphosphate reductase gene (nrdA) of Escherichia coli carries a repetitive extragenic palindromic (REP) sequence in its 3' structural terminus Molecular Microbiology, 3, 839-841.
Gosset,G. Merino,E. Recillas,F. Oliver,G. Becerril,B. Bolivar,F.
1989. Amino acid sequence analysis of the glutamate synthase enzyme from Escherichia coli K-12.Protein sequences & data analysis, 2, 9-16.
Bolivar,F.
1988. Plasmid pBR322: the multipurpose cloning vector.Focus, 10, 61-64.
Balbas,P. Soberon,X. Bolivar,F. Rodriguez,R.L.
1988. The plasmid, pBR322.Biotechnology (Reading, Mass.), 10, 5-41.
Stieglitz,H. Cervantes,L. Robledo,R. Fonseca,R. Covarrubias,L. Bolivar,F. Kupersztoch,Y.M.
1988. Cloning, sequencing, and expression in Ficoll-generated minicells of an Escherichia coli heat-stable enterotoxin gene Plasmid, 20, 42-53.
Riba,L. Becerril,B. Servin-Gonzalez,L. Valle,F. Bolivar,F.
1988. Identification of a functional promoter for the Escherichia coli gdhA gene and its regulation Gene, 71, 233-246.
Merino,E. Becerril,B. Valle,F. Bolivar,F.
1987. Deletion of a repetitive extragenic palindromic (REP) sequence downstream from the structural gene of Escherichia coli glutamate dehydrogenase affects the stability of its mRNA Gene, 58, 305-309.
Oliver,G. Gosset,G. Sanchez-Pescador,R. Lozoya,E. Ku,L.M. Flores,N. Becerril,B. Valle,F. Bolivar,F.
1987. Determination of the nucleotide sequence for the glutamate synthase structural genes of Escherichia coli K-12 Gene, 60, 1-11.
Balbas,P. Soberon,X. Merino,E. Zurita,M. Lomeli,H. Valle,F. Flores,N. Bolivar,F.
1986. Plasmid vector pBR322 and its special-purpose derivatives--a review Gene, 50, 3-40.
Valle,F. Gosset,G. Tenorio,B. Oliver,G. Bolivar,F.
1986. Characterization of the regulatory region of the Escherichia coli penicillin acylase structural gene Gene, 50, 119-122.
Flores,N. de Anda,R. Guereca,L. Cruz,N. Antonio,S. Balbas,P. Bolivar,F. Valle,F.
1986. A new expression vector for the production of fused proteins in Escherichia coli Applied Microbiology and Biotechnology, 25, 267-271.
Ladron de Guevara,O.L. Estrada,G. Antonio,S. Alvarado,X. Guereca,L. Zamudio,F. Bolivar,F.
1985. Identification and isolation of human insulin A and B chains by high-performance liquid chromatography Journal of Chromatography A, 349, 91-98.
Oliver,G. Valle,F. Rosetti,F. Gomez-Pedrozo,M. Santamaria,P. Gosset,G. Bolivar,F.
1985. A common precursor for the two subunits of the penicillin acylase from Escherichia coli ATCC11105 Gene, 40, 9-14.
Becerril,B. Valle,F. Merino,E. Riba,L. Bolivar,F.
1985. Repetitive extragenic palindromic (REP) sequences in the Escherichia coli gdhA gene Gene, 37, 53-62.
Zurita,M. Bolivar,F. Soberon,X.
1984. Construction and characterization of new cloning vehicles. VII. Construction of plasmid pBR327par, a completely sequenced, stable derivative of pBR327 containing the par locus of pSC101 Gene, 28, 119-122.
Valle,F. Becerril,B. Chen,E. Seeburg,P. Heyneker,H. Bolivar,F.
1984. Complete nucleotide sequence of the glutamate dehydrogenase gene from Escherichia coli K-12 Gene, 27, 193-199.
Valle,F. Sanvicente,E. Seeburg,P. Covarrubias,A. Rodriguez,R.L. Bolivar,F.
1983. Nucleotide sequence of the promoter and amino-terminal coding region of the glutamate dehydrogenase structural gene of Escherichia coli Gene, 23, 199-209.
Garciarrubio,A. Lozoya,E. Covarrubias,A. Bolivar,F.
1983. Structural organization of the genes that encode two glutamate synthase subunits of Escherichia coli Gene, 26, 165-170.
Covarrubias,L. Bolivar,F.
1982. Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication Gene, 17, 79-89 * .
Sanchez-Pescador,R. Sanvicente,E. Valle,F. Bolivar,F.
1982. Recombinant plasmids carrying the glutamate dehydrogenase structural gene from Escherichia coli K-12 Gene, 17, 1-8 * .
Villa,L.L. Bolivar,F. Renzo Brentani,R.
1981. Human insulin: Construction of a plasmid containing its coding sequences.Arquivos Brasileiros de Endocrinologia e Metabologia, 25, 61-64 * .
Covarrubias,L. Cervantes,L. Covarrubias,A. Soberon,X. Vichido,I. Blanco,A. Kupersztoch-Portnoy,Y.M. Bolivar,F.
1981. Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328 Gene, 13, 25-35 * .
Soberon,X. Covarrubias,L. Bolivar,F.
1980. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325 Gene, 9, 287-305 * .
Covarrubias,A.A. Rocha,M. Bolivar,F. Bastarrachea,F.
1980. Cloning and physical mapping of the glnA gene of Escherichia coli K-12 Gene, 11, 239-251 * .
Fuchs,L.Y. Covarrubias,L. Escalante,L. Sanchez,S. Bolivar,F.
1980. Characterization of a site-specific restriction endonuclease SphI from Streptomyces phaeochromogenes Gene, 10, 39-46 * .
Covarrubias,A.A. Sanchez-Pescador,R. Osorio,A. Bolivar,F. Bastarrachea,F.
1980. ColE1 hybrid plasmids containing Escherichia coli genes involved in the biosynthesis of glutamate and glutamine Plasmid, 3, 150-164 * .
Joseph-Bravo,P. Charli,J.L. Sherman,T. Boyer,H. Bolivar,F. McKelvy,J.F.
1980. Identification of a putative hypothalamic mRNA coding for somatostatin and of its product in cell-free translation Biochemical and Biophysical Research Communications, 94, 1004-1012 * .
Lozoya,E. Sanchez-Pescador,R. Covarrubias,A. Vichido,I. Bolivar,F.
1980. Tight linkage of genes that encode the two glutamate synthase subunits of Escherichia coli K-12 Journal of Bacteriology, 144, 616-621 * .
Goeddel,D.V. Kleid,D.G. Bolivar,F. Heyneker,H.L. Yansura,D.G. Crea,R. Hirose,T. Kraszewski,A. Itakura,K. Riggs,A.D.
1979. Expression in Escherichia coli of chemically synthesized genes for human insulin Proceedings of the National Academy of Sciences of the United States of America, 76, 106-110 * .
Rodriguez,R.L. West,R.W. Heyneker,H.L. Bolivar,F. Boyer,H.W.
1979. Characterizing wild-type and mutant promoters of the tetracycline resistance gene in pBR313 Nucleic Acids Research, 6, 3267-3287 * .
Bolivar,F.
1979. Molecular cloning vectors derived from the CoLE1 type plasmid pMB1 Life Sciences, 25, 807-817 * .
Greene,P.J. Heyneker,H.L. Bolivar,F. Rodriguez,R.L. Betlach,M.C. Covarrubias,A.A. Backman,K. Russel,D.J. Tait,R. Boyer,H.W.
1978. A general method for the purification of restriction enzymes Nucleic Acids Research, 5, 2373-2380 * .
Bolivar,F.
1978. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules Gene, 4, 121-136 * .
Bolivar,F. Rodriguez,R.L. Betlach,M.C. Boyer,H.W.
1977. Construction and characterization of new cloning vehicles. I. Ampicillin-resistant derivatives of the plasmid pMB9 Gene, 2, 75-93 * .
Bolivar,F. Rodriguez,R.L. Greene,P.J. Betlach,M.C. Heyneker,H.L. Boyer,H.W.
1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system Gene, 2, 95-113 * .
Bolivar,F. Betlach,M.C. Heyneker,H.L. Shine,J. Rodriguez,R.L. Boyer,H.W.
1977. Origin of replication of pBR345 plasmid DNA Proceedings of the National Academy of Sciences of the United States of America, 74, 5265-5269 * .
Itakura,K. Hirose,T. Crea,R. Riggs,A.D. Heyneker,H.L. Bolivar,F. Boyer,H.W.
1977. Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin Science, 198, 1056-1063 * .
Bolivar,F. Covarrubias,A. Rodriguez,R.
1976. Clonación de ADN de N. crassa y E. coli utilizando tres enzimas de restricción y un vehículo molecular con sitios específicos para cada una de ellas. Revista de la Sociedad Química de México, 20, 260-264 * .
Sanchez,F. Bolivar,F. Martuscelli,J.
1976. Transformation of Escherichia coli by chromosomal deoxyribonucleic acid from Salmonella typhi Journal of Bacteriology, 125, 747-749 * .
Bolivar,F. Zetina,M.E. Martuscelli,J.
1976. [Phenotypic suppression of thermosensitive mutations to the initiation of DNA synthesis by the integretion of an antibiotic-resistant factor].Boletin de estudios medicos y biologicos, 28, 403-409 * .
Sanchez,F. Bolivar,F. Martuscelli,J.
1975. Transformation of Escherichia coli K-12 by linear DNA from Salmonella typhi.Microbial genetics bulletin, 38, 13-14 * .
Martuscelli,J. Bolivar,F. Ramirez-Bon,E. Canedo,L.
1973. [Various physicochemical properties of the bacteriophage Mu-1].Boletin de estudios medicos y biologicos, 28, 33-40 * .
Libros y capítulos recientes Escalante,A. Lopez-Soto,D.R. Velazquez-Gutierrez,J.E. Giles-Gomez,M. Bolivar,F. Lopez-Munguia,A. 2019. Pulque, a Traditional Mexican Alcoholic Fermented Beverage: Historical, Microbiological, and Technical Aspects [reimpresión Front Microbiol. 2016 Jun 30;7:1026] en: Shin,D.H. Microbiology of Ethnic Fermented Foods and Alcoholic Beverages of the World.. Lausanne. Frontiers Media.
Bolivar,F. 2012. Oportunidades y retos con el manejo responsable de los organismos geneticamente modificados en: Ciencia y humanismo. Memorias. Academia Mexicana de Ciencias. pags. 493-503
Bolivar-Zapata,F.G. Arias-Ortiz,C.F. Arriaga-Arellano,E. Barrera-Saldaña,H. de la Torres-Martinez,M. Espinosa-Fernandez,J. Galindo-Fentanes,E. Galvez-Mariscal,A. Gracia-Gasca,A. Herrera-Estrella,L. Larque-Saavedra,A. Lopez-Munguia Canales,A. Noyola-Robles,A. Paredes-Lopez,O. Ramirez-Reivich,O.T. Revah-Moiseev,S. Soberon-Mainero,J. Soberon-Mainero,X. Torres-Pacheco,I. Uribe de la Mora,J. Viniegra-Gonzalez,G. 2011. Por un uso responsable de los organismos genéticamente modificados Mexico:. Academia Mexicana de las Ciencias.
Bolivar-Zapata,F. 2010. Ciencias Biológicas y de la Salud: Cuatro visiones y un mismo compromiso en: Zuniga-Bello,P. El Debate de la Ciencia en México.. Foro Consultivo Científico y Tecnológico. pags. 37-43
Bolivar,F. 2010. La Biotecnologia: su impacto y desarrollo en Mexico en: Matos Moctezuma,E. El Pasado del Presente. Miradas y Perspectivas 1810-1910. Mexico. El Colegio Nacional. pags. 267-309
Bolivar,F. 2010. La UNAM en el avance de la Ciencia y la descentralización de la educación superior y la investigación. en: Martuscelli Quintana,J. Guillermo Soberón Acevedo, Su Impacto en la Ciencia, la Educación Superior y la Salud.. Mexico. UNAM. pags. 29-44
Bolivar,F. 2008. Diseño y construcción de herramientas moleculares para la clonación y expresión de material genético: la construcción de las primeras bacterias transgénicas productoras de proteínas humanas de interés médico en: Estrada-Orihuela,S. Aportaciones científicas y humanísticas mexicanas en el siglo XX. Mexico. Fondo de Cultura Economica. pags. 85-91
Bolivar-Zapata,F.G. 2008. Creación y consolidación del Centro de Investigación sobre Ingeniería Genética y Biotecnología y su transformación en el Instituto de Biotecnología de la UNAM en: *agustin Una ventana al quehacer científico. Instituto de Biotecnología de la UNAM 25 aniversario, cap 1. Mexico, D.F.. UNAM. pags. 13-20
Bolivar-Zapata,F.G. 2007. Biotecnología y bioseguridad para el desarrollo de México. La pertinencia de la Ley de Bioseguridad en: Brena-Sesma,I. Panorama Internacional en Salud y Derecho. Culturas y Sistemas Jurídicos Comparados. Mexico. IIJ-UNAM. pags. 21-28
Arriaga,E. Bolivar,F. 2007. Por un uso responsable de los Organismos Genéticamente Modificados en: *bolivar Fundamentos y casos exitosos de la Biotecnología Moderna.. Mexico. El Colegio Nacional. pags. 675-694
Bolivar-Zapata,F.G. 2007. Por un uso responsable de los organismos genéticamente modificados Mexico:. Academia Mexicana de las Ciencias.
Bolivar-Zapata,F.G. 2007. Fundamentos y casos exitosos de la biotecnologia moderna Mexico:. El Colegio Nacional.
Bolivar,F. 2006. La biotecnologia en Mexico en: Simposio sobre Eduacion Superior. Mexico. El Colegio Nacional. pags. 277-287
Chavez-Bejar,M. Martinez,A. Bolivar,F. Gosset,G. 2005. Metabolic pathway engineering for microbial production of aromatic amino acids en: Moham,R.M. Research Advances in Food Science. Kerala. Global Research network. pags. 11-20
Bolivar,F. 2005. Marco juridico en biotecnologia y bioseguridad en Mexico. La ley de bioseguridad para organismos geneticamente modificados aprobada por el Senado de la Republica en: Diaz-Muller,L.T. Segundas jornadas sobre globalizacion y derechos humanos. Mexico. pags. 1-12
Le Borgne,S. Bolivar,F. Gosset,G. 2004. Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli en: Lorence,A. Recombinant gene expression : reviews and protocols. pags. 135-144
Balbas,P. Bolivar,F. 2004. Back to Basics: pBR322 and Protein Expression Systems in E. coli en: Lorence,A. Recombinant Gene Expression Reviews and Protocols. Springer. pags. 77-90
Bolivar,F. 2004. Moleculas informacionales de la celula viva. Acidos nucleicos y proteinas. en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 19-53
Bolivar,F. 2004. Ingenieria genetica las herramientas moleculares y los metodos para aislar, caracterizar y manipular el DNA. en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 57-83
Gosset,G. Bolivar,F. 2004. Ingenieria celular microbiana en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 219-247
Bolivar,F. 2004. Ciencia genomica, proteomica y bioinformatica. El genoma, el transcriptoma y el proteoma humano en: *bolivar Fundamentos y casos exitosos de la biotecnologia moderna. Mexico. El Colegio Nacional. pags. 84-113
Bolivar,F. 2004. Fundamentos y casos exitosos de la biotecnologia moderna Mexico:. El Colegio Nacional.
Galindo,E. Larque,A. Arriaga,E. Bolivar,F. 2003. Situación de la Biotecnología en México en: Pena,J.A. Estado Actual y Prospectiva de la Ciencia en México. Mexico,D.F.. Academia Mexicana de Ciencias. pags. 62-91
Cantu,J.M. Garcia,J.E. Bolivar,F. 2003. El genoma humano en: *bolivar Fronteras de la Biología en los Inicios del Siglo XXI, Módulo I. Mexico,D.F:. El Colegio Nacional. pags. 143-154
Bolivar,F. 2003. De la genética a la genómica en: *bolivar Fronteras de la Biología en los Inicios del Siglo XXI, Módulo I.. Mexico,D.F.. El Colegio Nacional. pags. 7-54
Gosset,G. Bolivar,F. 2003. Ingeniería celular en microorganismos en: *agustin Fronteras de la Biología en los Inicios del Siglo XXI. Módulo VII. Mexico,D.F:. El Colegio Nacional, México. pags. 5-34
Bolivar,F. Lopez,S. Arias,C.F. Puente,J.L. Palacios,R. Davila,G. Flores,M. Gonzalez,A. de Luna,A. Anaya,V. Covarrubias,A. Cantu,J.M. Garcia,J.E. Collado,J. Medrano,A. 2003. Fronteras de la Biología en los Inicios del Siglo XXI: Genómica, Proteómica y Bioinformática. Mexico, D.F.: El Colegio Nacional.
Gosset,G. Bolivar,F. Soberon,X. Lopez-Munguia,A. Valderrama,B. Ayala,M. Vazquez,R. Ramirez,O.T. Alagon,A. Possani,L.D. 2003. Fronteras de la Biología en los Inicios del Siglo XXI. Módulo VII: Ingeniería Celular, Biodiversidad e Industria. Mexico, D.F.: El Colegio Nacional.
Bolivar,F. Herrera-Estrella,L. Villelle,J.Ph. Estrada,A. Garcia,M. 2003. Fronteras de la Biología en los Inicios del Siglo XXI. Módulo III: Biotecnología Agrícola. Mexico, D.F: El Colegio Nacional.
Arias,C.F. Arriaga,E. Barrera,H. Bolivar,F. Bosch,P. De la Torre,M. Espinosa,J. Galindo,E. Galvez,A. Garcia,A. Herrera,L.R. Larque,A. Lopez-Munguia,A. Loyola,A. Ortega,R. Paredes.O. Ramirez,O.T. Revah,S. Serratos,J.A. Soberon,J. Soberon,X. Torres,I. Uribe,J. Viniegra,G. 2003. Recomendaciones para el Desarrollo y Consolidación de la Biotecnología en México Mexico,D.F.: Academia Mexicana de Ciencias, A.C..
Bolivar,F. 2002. Comentarios a la ley para el fomento de la investigación científica y tecnológica en: Valades,D. Investigación y Desarrollo en la Reforma Fiscal. Mexico, D.F:. Academia Mexicana de Ciencias-UNAM. pags. 23-28
Bolivar,F. Lopez-Munguia,A. 2002. Biotecnología moderna y alimentos transgénicos: mitos y realidades en: Bourges-Rodriguez,H. La Alimentación de los Mexicanos. Mexico. D.F.. El Colegio Nacional. pags. 153-173
Bolivar,F. 2002. Reflexiones sobre Ciencia y Tecnología. Mexico, D.F: El Colegio Nacional.
Bolivar,F. 2002. Biotecnologia moderna para el desarrollo de Mexico en el siglo XXI: retos y oportunidades. Conacyt.
Bolivar,F. 2001. Moleculas informacionales y el origen de la vida en: Rudomin,P. Una vision integradora: universo, vida, hombre y sociedad. Mexico. El Colegio Nacional. pags. 117-153
Soberon,X. Bolivar,F. 2001. Evolucion de los conceptos de gen y genoma en: Blazquez-Graf,N. Ciencias de la vida. Siglo XXI. pags. 61-83
Bolivar,F. Rudomin,P. 2001. Una Visión Integradora; Universo, Vida, Hombre y Sociedad. Mexico, D.F.: El Colegio Nacional.
Bolivar,F. 2001. La Genética Moderna; Fundamentos y Horizontes. Mexico, D.F.: El Colegio Nacional.
Balbas,P. Bolivar,F. 1998. Molecular cloning by plasmid vectors en: Rao,V.B. Recombinant DNA principles and methodologies. New York. CRC Press. pags. 383-411
Gomez,A. Rodriguez,M. Ospina,S. Zamora,R. Merino,E. Bolivar,F. Ramirez,O.T. Quintero,R. Lopez-Munguia,A. 1994. Strategies in the design of a penicillin acylase process en: *tonatiuh Kluwer Academic. pags. 29-40
Balbas,P. Bolivar,F. 1990. [3] Design and construction of expression plasmid vectors in Escherichia coli en: Goeddel,D.V. Methods in Enzymology. pags. 14-37
Balbas,P. de Anda,R. Flores,N. Alvarado,X. Cruz,N. Valle,F. Bolivar,F. 1988. Overproduction of proteins by recombinant DNA: human insulin en: Jaz,J. Cell Function and Disease. New York. Plenum. pags. 59-74
Covarrubias,A. Sanchez-Pescador,R. Lozoya,E. Bastarrachea,F. Bolivar,F. 1980. Isolation of E. coli Chromosomal DNA Regions Carrying glnA, gdh and gltB Structural Genes en: Palacios,R. Glutamine: Metabolism, Enzymology, and Regulation. Academic Press. pags. 123-137 *
Bastarrachea,F. Brom,S. Covarrubias,A.A. Osorio,A. Bolivar,F. 1980. Genetic Characterization of Mutations Affecting Glutamine Biosynthesis and its Regulation in Escherichia coli K12 en: Palacios,R. Glutamine: Metabolism, Enzymology, and Regulation. Academic Press. pags. 107-121 *
Riggs,A.D. Itakura,K. Crea,R. Hirose,T. Kraszewski,A. Goeddel,D. Kleid,D. Yansura,D.G. Bolivar,F. Heyneker,H.L. 1980. Synthesis, Cloning, and Expression of Hormone Genes in Escherichia coli en: Greep,R.O. Recent Progress in Hormone Research Proceedings of the 1979 Laurentian Hormone Conference. Boston. Academic Press. pags. 261-276 *
Bolivar,F. Backman,K. 1979. [16] Plasmids of Escherichia coli as cloning vectors en: Wu,R. Methods in Enzymology. pags. 245-267
Tait,R.C. Heyneker,H.L. Bolivar,F. Rodriguez,A. Covarrubias,A.A. Betlach,M. Boyer,H.W. 1978. Tetracycline resistance conferred by pSC101, pMB9 and their derivatives en: Microbiology-1978, American Society for Microbiology. pags. 174-176 *
Bolivar,F. 1978. Molecular cloning vectors: derivatives of plasmid pBR322 en: Nicosia,S. Elsevier. pags. 59-64 *
Rodriguez,R.L. Tait,R.C. Shine,J. Bolivar,F. Heyneker,H. Betlach,M. Boyer,H.W. 1977. Characterization of tetracycline and ampicillin resistant plasmid cloning vehicles en: Werner,R. New York. Academic Press. pags. 73-78 *
Boyer,H.W. Betlach,M. Bolivar,F. Rodriguez,R.L. Heyneker,H.L. Shine,J. Goodman,H.M. 1977. The construction of molecular cloning vehicles en: Roland,F.B. New York. Raven Press. pags. 9-20 *
Rodriguez,R.L. Bolivar,F. Goodman,H.M. Boyer,H.W. Betlach,M. 1976. Construction and characterization of cloning vehicles en: Fox,C.F. Molecular mechanisms in the control of gene expression : [proceedings]. New York. Academic Press. pags. 471-477 *
Otras Publicaciones Mendoza-Flores,R. Diaz-Quiroz,D. Sabido-Ramos,A. Bolivar,F. Escalante,A.
2020. Ingeniería de vías metabólicas en bacterias para la producción de intermediarios de interés industrial de la vía del aminoshikimato Biotecnologia, 24, 11-28.
Alva,A. Bolivar,F. Escalante,A.
2017. Evaluación de Pseudomonas chlororaphis como nuevo modelo para la producción de ácido shikímico. Biotecnologia, 21, 66-79.
Diaz,D.C. Bolivar,F. Escalante,A.
2016. Ingeniería de Proteínas Como una Herramienta en Ingeniería de Vías Metabólicas Biotecnologia, 20, 16-34.
Carmona,S. Bolivar,F. Escalante,A.
2013. Efecto de la Clonación del Gen zwf sobre la Producción de Shikimato en la Cepa de Escherichia coli PB12.SA22 Biotecnologia, 17, 66-86.
Sabido,A. Martinez,A. Bolivar,F. Gosset,G.
2011. Generación de un Sistema para la Integración y Expresión de Genes en el Cromosoma de Escherichia coli Biotecnologia, 15, 35-49.
Cortes-Tolalpa,L. Carmona,S.B. Cervantes-Salinas,A. Garcia,M.A. Gosset,G. Escalante,A. Bolivar,F.
2011. Ingeniería de vías metabólicas en Escherichia coli para la producción de Shikimato como precursos para la síntesis de compuestos antivirales contra influenza. Biotecnologia, 15, 30-47.
Bolivar-Zapata,F.
2009. Espacio esencial en México Revista Universidad de Mexico, 68, 7-9.
Bolivar-Zapata,F.
2009. La Universidad Pública: Espacio vital para la nación mexicana Revista Universidad de Mexico, 59, 24-29.
Escalante,A. Valdivia,A. Gosset,G. Bolivar,F.
2009. Ingeniería de Vías Metabólicas en Escherichia Coli Pts– para la Obtención de Cepas Sobreproductoras de Shikimato Mensaje Bioquimico, 33, 171-180.
Baez-Viveros,J.L. Osuna,J. Hernandez-Chavez,G. Soberon,X. Bolivar,F. Gosset,G.
2006. Metabolic via engineering for the production of aromatic substances in Escherichia coli Revista Latinoamericana de Microbiologia, 48, 92.
Huerta-Beristain,G. Utrilla,J. Hernandez-Chavez,G. Bolivar,F. Martinez,A.
2005. Ingenieria metabolica para incrementar el flux y rendimientos de etanol en Escherischia coli etanologenica Revista Mexicana de Ingenieria Quimica, 4, 25-36.
Escalante-Lozada,A. Gosset-Lagarda,G. Martinez-Jimenez,A. Bolivar-Zapata,F.
2004. Soil bacterial diversity: Microbial culture-independent methods of study and biotechnological implications Agrociencia, 38, 583-592.
Bolivar,F.
2003. La pertinencia de la Ley de Bioseguridad de OGM.Scientific American Mexico, 13, 23.
Martinez,A. Bolivar,F. Gosset,G.
2002. Biotecnologia energetica sustentable: etanol carburante para el transporte Revista Universidad de Mexico, 617, .
Bolivar,F.
2002. Biotecnologia moderna para el desarrollo de Mexico Este Pais: tendencias y opiniones, 140, 56-59.
Bolivar,F.
2002. Propuesta de creacion del Centro de Investigacion sobre Ingenieria Genetica y Biotecnologia de la UNAM Revista Universidad de Mexico, 617, 1.
Meagher,L.R. Bolivar,F.
1998. Changing university roles in the century of biotechnology Nature Biotechnology, 16, 598-599.
Munoz,M.E. Le Borgne,S. Bolivar,F. Valle,F.
1997. Molecular cloning of the gene that codes for the pyruvate kinase of Bacillus subtilis: primary characterization of a strain carrying this gene insertionally inactivated Revista Latinoamericana de Microbiologia, 39, 129-140.
Oliver,G. Balbas,P. Valle,F. Soberon,X. Bolivar,F.
1985. [Cloning of human leukocyte interferon cDNA and a strategy for its production in E. coli].Revista Latinoamericana de Microbiologia, 27, 141-150.
Sanvicente,E. Sanchez-Pescador,R. Valle,F. Bolivar,F.
1983. [Biochemical evidence of the presence of a structural gene of glutamic dehydrogenase in E. coli K-12 in recombinant plasmids].Boletin de estudios medicos y biologicos, 32, 225-232.
Vichido,I. Bolivar,F.
1982. [Molecular cloning of DNA complementary to messenger RNA coding for rat preproinsulin].Boletin de estudios medicos y biologicos, 32, 13-29 * .
Bolivar-Zapata,F.G.
1979. Recombinacion in vitro de acidos nucleicos: ingenieria genetica molecular.Revista Latinoamericana de Microbiologia, 21, 37-55 * .
Patentes José Manuel Camacho Zaragoza, Georgina Teresa Hernández Chávez, Fabián Moreno Avitia, René Ramírez Iñiguez, Alfredo Martínez Jiménez , Francisco Bolívar Zapata, Guillermo Gosset Lagarda 2017 Cepas de Escherichia coli modificadas por ingeniería de vías metabólicas para la biosíntesis de resveratrol y su uso en un nuevo proceso de co-cultivo.UNAM México. (en trámite)
Ponce Laura Grecia , Martínez Mejía Luz María , Ramírez Reivich Octavio Tonatiuh , Martínez Jiménez Alfredo , Bolívar Zapata Francisco Gonzalo , Gosset Lagarda Guillermo . 2013 Cepas de Escherichia coli con alta capacidad para la producción de plásmidos y su uso en la producción de vacunas de ADN.UNAM México. (en trámite)
Guillermo Gosset Lagarda , Alfredo Martínez Jiménez , Francisco Gonzalo Bolívar Zapata , Ana Joyce Muñoz Arellano, Georgina Teresa Hernández Chávez y Ramón de Anda Herrera 2011 Cepas de Escherichia coli modificadas para producir 3,4-hidroxi-L-fenilalanina (L-Dopa).UNAM México. (en trámite)
A. R. Lara R., M. de C. Vázquez L., G. Gosset L., F. G. Bolívar Z., A. Lopez-Munguia C. y O. T. Ramírez R 2006 Estrategia para generar células insensibles a condiciones heterogéneas en biorreactores industriales a través de mutaciones de vías metabólicas anaerobias.UNAM México. (en trámite)
J. Osuna, J.L. Báez, G. Hernández, F. G. Bolivar, X. Soberón y G. Gosset 2005 Versiones insensibles a inhibición alostérica y catalíticamente eficientes de la enzima corismato mutasa-prefenato deshidratasa y su aplicación para la producción de L-fenilalanina en microorganismos.
G. Gosset, A. Martínez, F. G. Bolívar, V. H. Lagunas, N. Cabrera, J. G. Dávila, V. M. González y V. P. Bustos 2004 Producción de melaninas en microorganismos recombinantes.UNAM México. (en trámite)
L. T. Casas T. F. Bastarrachea A. R. Quintero R. J. D. Carranco R. E. Galindo F. F. G. Bolívar Z. 1995 Proceso para producir la enzima penicilino amidasa en células de E. coli.UNAM México.
F. G. Bolívar Z. G. Gosset L. R. de Anda R. Quintero R. A. Martínez F. Valle N. Flores M. 1994 Proceso fermentativo para obtener proteínas híbridas a partir de cepas de Escherichia coli .UNAM México.
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