. Instituto de Biotecnologia UNAM

IBT-UNAM : Dr. Takuya Nishigaki

Dr. Takuya Nishigaki

Líder Académico »» Grupo
Investigador
Tutor de Maestría y Doctorado
Nivel II del SNI
Departamento de Genética del Desarrollo y Fisiología Molecular

Líneas de investigación

email: takuya @ibt.unam.mx
Teléfonos
Oficina +52 (777) 329-1709
desde la CDMX 562-27709
red UNAM 27709

Laboratorio +52 (777) 329-1777 ext 38133.
desde la CDMX 562-27777 ext 38133
red UNAM 38133

- Licenciatura: Ciencias, Instituto Tecnologico de Tokyo (1992)
- Maestría: en Biociencias, Instituto Tecnologico de Tokyo (1994)
- Doctorado: en Biociencias, Instituto Tecnologico de Tokyo (1997)
- Estancia de investigacion en el laboratorio del Dr. Alberto Darszon, del IBt-UNAM (1997-1999)

Estudiantes

Columba Alejandra López

Biol. César Arcos

M.C. Sandra Hernández

M.C. Edith Odemaris López

Publicaciones recientes

De La Rosa,J.A.M. GarcIa-Castaneda,M. Nishigaki,T. Gomora,J.C. Mancilla-Percino,T. Avila,G. 2020. Interaction of MDIMP with the Voltage-Gated Calcium Channels Molecular Pharmacology, 98, 211-221.

Garza-Lopez,E. Aldana,A. Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. 2020. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in its domains I/II Cell Calcium, 89, 102214.

Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. Visconti,P.E. Trevino,C.L. 2020. Differences and Similarities: The Richness of Comparative Sperm Physiology Physiology (Bethesda.), 35, 196-208.

Priego-Espinosa,D.A. Darszon,A. Guerrero,A. Gonzalez-Cota,A.L. Nishigaki,T. Martinez-Mekler,G. Carneiro,J. 2020. Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm PLoS Computational Biology, 16, e1007605.

Chavez,J.C. Darszon,A. Trevino,C.L. Nishigaki,T. 2020. Quantitative intracellular pH determinations in single live mammalian spermatozoa using the ratiometric dye SNARF-5F Frontiers in Cell and Developmental Biology, 7, 366.

Romero,F. Nishigaki,T. 2019. Comparative genomic analysis suggests that the sperm-specific sodium/proton exchanger and soluble adenylyl cyclase are key regulators of CatSper among the Metazoa Zoological Letters, 5, 25.

Kinoshita-Terauchi,N. Shiba,K. Terauchi,M. Romero,F. Ramirez-Gomez,H.V. Yoshida,M. Motomura,T. Kawai,H. Nishigaki,T. 2019. High potassium seawater inhibits ascidian sperm chemotaxis, but does not affect the male gamete chemotaxis of a brown alga Zygote, 27, 225-231.

Santana-Calvo,C. Romero,F. Lopez-Gonzalez,I. Nishigaki,T. 2018. Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor Biotechniques, 65, 211-218.

Chavez,J.C. de la Vega-Beltran,J.L. Jose,O. Torres,P. Nishigaki,T. Trevino,C.L. Darszon,A. 2018. Acrosomal alkalization triggers Ca2+ release and acrosome reaction in mammalian spermatozoa Journal of Cellular Physiology, 233, 4735-4747.

Romero,F. Santana-Calvo,C. Sanchez-Guevara,Y. Nishigaki,T. 2017. FRET-based binding assay between a fluorescent cAMP analogue and a cyclic nucleotide-binding domain tagged with a CFP FEBS Letters, 591, 2869-2878.

Nishigaki,T. Romero,F. Sanchez-Guevara,Y. 2016. CatSper, el canal de Ca2+ que regula el batido flagelar del espermatozoide en eucariontes Revista Iberoamericana de Ciencias, 3, 28-36.

Gijsbers,A. Nishigaki,T. Sanchez-Puig,N. 2016. Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions Journal of Visualized Experiments JoVE, 116, .

Garza-Lopez,E. Chavez,J.C. Santana-Calvo,C. Lopez-Gonzalez,I. Nishigaki,T. 2016. Cd2+ sensitivity and permeability of a low voltage-activated Ca2+ channel with CatSper-like selectivity filter Cell Calcium, 60, 41-50.

Arcos-Hernandez,C. Romero,F. Sanchez-Guevara,Y. Beltran,C. Nishigaki,T. 2016. FRET analysis using sperm-activating peptides tagged with fluorescent proteins reveals that ligand-binding sites exist as clusters Journal of Experimental Biology, 219, 508-515.

Garza-Lopez,E. Sanchez-Carranza,O. Nishigaki,T. Lopez-Gonzalez,I. 2015. Pharmacological identification of endogenous slo1 channel-b1 subunit complexes in CHO cells using three aKTx1 subfamily toxins International Journal of Pharmacy & Therapeutics, 6, 11-24.

Beltran,C. Rodriguez-Miranda,E. Granados-Gonzalez,G. de De la Torre LG Nishigaki,T. Darszon,A. 2014. Zn induces hyperpolarization by activation of a K channel and increases intracellular Ca and pH in sea urchin spermatozoa Developmental Biology, 394, 15-23.

Nishigaki,T. Jose,O. Gonzalez-Cota,A.L. Romero,F. Trevino,C.L. Darszon,A. 2014. Intracellular pH in Sperm Physiology Biochemical and Biophysical Research Communications, 450, 1149-1158.

Sanchez-Tusie,A.A. Vasudevan,S.R. Churchill,G.C. Nishigaki,T. Trevino,C.L. 2014. Characterization of NAADP-Mediated Calcium Signaling in Human Spermatozoa Biochemical and Biophysical Research Communications, 443, 531-536.

Servin-Vences,M.R. Tatsu,Y. Ando,H. Guerrero,A. Yumoto,N. Darszon,A. Nishigaki,T. 2012. A caged progesterone analog alters intracellular Ca2+ and flagellar bending in human sperm Reproduction, 144, 101-109.

Darszon,A. Nishigaki,T. Beltran,C. Trevino,C.L. 2011. Calcium Channels in the Development, Maturation, and Function of Spermatozoa Physiological Reviews, 91, 1305-1355.

Chavez,J.C. de Blas,G.A. de la Vega-Beltran,J.L. Nishigaki,T. Chirinos,M. Gonzalez-Gonzalez,M.E. Larrea,F. Solis,A. Darszon,A. Trevino,C.L. 2011. The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa: differences from the zona pellucida Asian Journal of Andrology, 13, 159-165.

Guerrero,A. Wood,C.D. Nishigaki,T. Carneiro,J. Darszon,A. 2010. Tuning sperm chemotaxis Biochemical Society Transactions, 38, 1270-1274.

Guerrero,A. Nishigaki,T. Carneiro,J. Tatsu,Y. Wood,C.D. Darszon,A. 2010. Tuning sperm chemotaxis by calcium burst timing Developmental Biology, 344, 52-65.

Darszon,A. Guerrero,A. Galindo,B.E. Nishigaki,T. Wood,C.D. 2008. Sperm-activating peptides in the regulation of ion fluxes, signal transduction and motility International Journal of Developmental Biology, 52, 595-606.

de la Sancha,C.U. Martinez-Cadena,G. Lopez-Godinez,J. Castellano,L.E. Nishigaki,T. Darszon,A. Garcia-Soto,J. 2007. Rho-kinase (ROCK) in sea urchin sperm: Its role in regulating the intracellular pH during the acrosome reaction Biochemical and Biophysical Research Communications, 364, 470-475 [Corrigendum en Volume 366, Issue 2, 2008, Page 604].

Darszon,A. Trevino,C.L. Wood,C. Galindo,B. Rodriguez-Miranda,E. Acevedo,J.J. Hernandez-Gonzalez,E.O. Beltran,C. Martinez-Lopez,P. Nishigaki,T. 2007. Ion channels in sperm motility and capacitation.Society of Reproduction and Fertility supplement, 65, 229-244.

Wood,C.D. Nishigaki,T. Tatsu,Y. Yumoto,N. Baba,S.A. Whitaker,M. Darszon,A. 2007. Altering the speract-induced ion permeability changes that generate flagellar Ca(2+) spikes regulates their kinetics and sea urchin sperm motility Developmental Biology, 306, 525-537.

Rangel-Mata,F. Mendez-Marquez,R. Martinez-Cadena,G. Lopez-Godinez,J. Nishigaki,T. Darszon,A. Garcia-Soto,J. 2007. Rho, Rho-kinase, and the actin cytoskeleton regulate the Na(+)-H(+) exchanger in sea urchin eggs Biochemical and Biophysical Research Communications, 352, 264-269.

Nishigaki,T. Wood,C.D. Shiba,K. Baba,S.A. Darszon,A. 2006. Stroboscopic illumination using light-emitting diodes reduces phototoxicity in fluorescence cell imaging Biotechniques, 41, 191-197.

Darszon,A. Acevedo,J.J. Galindo,B.E. Hernandez-Gonzalez,E.O. Nishigaki,T. Trevino,C.L. Wood,C. Beltran,C. 2006. Sperm channel diversity and functional multiplicity Reproduction, 131, 977-988.

Trevino,C.L. de la Vega-Beltran,J.L. Nishigaki,T. Felix,R. Darszon,A. 2006. Maitotoxin potently promotes Ca(2+) influx in mouse spermatogenic cells and sperm, and induces the acrosome reaction Journal of Cellular Physiology, 206, 449-456.

Wood,C.D. Nisihigaki,T. Furuta,T. Baba,S.A. Darszon,A. 2005. Real-time analysis of the role of Ca2+ in flagellar movement and motility in single sea urchin sperm Journal of Cell Biology, 169, 725-731 [correction J Cell Biol 170 (7): 1171-1171 SEP 26 2005].

Darszon,A. Nishigaki,T. Wood,C. Trevino,C.L. Felix,R. Beltran,C. 2005. Calcium channels and ca(2+) fluctuations in sperm physiology International Review of Cytology, 243, 79-172.

Shiba,K. Ohmuro,J. Mogami,Y. Nishigaki,T. Wood,C.D. Darszon,A. Tatsu,Y. Yumoto,N. Baba,S.A. 2005. Sperm-activating Peptide induces asymmetric flagellar bending in sea urchin sperm Zoological Science, 22, 293-299.

Darszon,A. Wood,C.D. Beltran,C. Sanchez,D. Rodriguez,E. Gorelik,J. Korchev,Y.E. Nishigaki,T. 2004. Measuring ion fluxes in sperm Methods in Cell Biology, 74, 545-576.

Nishigaki,T. Wood,C.D. Tatsu,Y. Yumoto,N. Furuta,T. Elias,D. Shiba,K. Baba,S.A. Darszon,A. 2004. A sea urchin egg jelly peptide induces a cGMP-mediated decrease in sperm intracellular Ca(2+) before its increase Developmental Biology, 272, 376-388.

Tatsu,Y. Nishigaki,T. Darszon,A. Yumoto,N. 2002. A caged sperm-activating peptide that has a photocleavable protecting group on the backbone amide FEBS Letters, 525, 20-24.

Darszon,A. Beltran,C. Felix,R. Nishigaki,T. Trevino,C.L. 2001. Ion Transport in Sperm Signaling Developmental Biology, 240, 1-14.

Nishigaki,T. Zamudio,F.Z. Possani,L.D. Darszon,A. 2001. Time-Resolved Sperm Responses to an Egg Peptide Measured by Stopped-Flow Fluorometry Biochemical and Biophysical Research Communications, 284, 531-535.

Nishigaki,T. Chiba,K. Hoshi,M. 2000. A 130-kDa membrane protein of sperm flagella is the receptor for asterosaps, sperm-activating peptides of starfish Asterias amurensis Developmental Biology, 219, 154-162 * .

Nishigaki,T. Darszon,A. 2000. Real-time measurements of the interactions between fluorescent speract and its sperm receptor Developmental Biology, 223, 17-26.

Galindo,B.E. Nishigaki,T. Rodriguez,E. Sanchez,D. Beltran,C. Darszon,A. 2000. Speract-receptor interaction and the modulation of ion transport in Strongylocentrotus purpuratus sea urchin sperm Zygote, 8, S20-S21.

Hoshi,M. Nishigaki,T. Kawamura,M. Ikeda,M. Gunaratne,J. Ueno,S. Ogiso,M. Moriyama,H. Matsumoto,M. 2000. Acrosome reaction in starfish: signal molecules in the jelly coat and their receptors Zygote, 8, S26-S27 * .

Darszon,A. Labarca,P. Nishigaki,T. Espinosa,F. 1999. Ion channels in sperm physiology Physiological Reviews, 79, 481-510.

Matsumoto,M. Briones,A.V. Nishigaki,T. Hoshi,M. 1999. 3.0.CO;2-8> Sequence analysis of cDNAs encoding precursors of starfish asterosaps Dev.Genet., 25, 130-136 * .

Byrne,M. Cerra,A. Nishigaki,T. Hoshi,M. 1997. Infestation of the testes of the Japanese sea star Asterias amurensis by the ciliate Orchitophyra stellarum: A caution against the use of this ciliate for biological control Diseases Of Aquatic Organisms, 28, 235-239 * .

Nishigaki,T. Chiba,K. Miki,W. Hoshi,M. 1996. Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs Zygote, 4, 237-245 * .

Hoshi,M. Nishigaki,T. Ushiyama,A. Okinaga,T. Chiba,K. Matsumoto,M. 1994. Egg-jelly signal molecules for triggering the acrosome reaction in starfish spermatozoa International Journal of Developmental Biology, 38, 167-174 * .

Nishigaki,T. Gonzalez-Cota,A.L. Orta Salazar,G.J. 2014. CatSper in Male Infertility en: Koschak,A. Pathologies of Calcium Channels. Springer Berlin Heidelberg. pags. 713-728

Nishigaki,T. Trevino,C.L. 2012. Study of protein-protein interactions by fluorescence spectroscopy en: *isabelg Tools to Understand Protein-Protein Interactions. Kerala. Transworld Research Network. pags. 87-100

Otras Publicaciones

Garza-Lopez,E. Aldana,A. Darszon,A. Nishigaki,T. Lopez-Gonzalez,I. 2019. CaV3.1 channel pore pseudo-symmetry revealed by selectivity filter mutations in their domains I/II bioRxiv, Preprint posted May 02, 2019, 626069.

Priego-Espinosa,D.A. Darszon,A. Guerrero,A. Gonzalez-Cota,A.L. Nishigaki,T. Martinez-Mekler,G. Carneiro,J. 2018. Modular mathematical analysis of the control of flagellar Ca2+-spike trains produced by CatSper and Cav channels in sea urchin sperm bioRxiv, preprint posted October 18, 2018, .

* Indica publicación con otra institución de adscripción